The PIVKA-II ELISA Kit is specifically designed for the precise and reliable detection of PIVKA-II levels in human serum samples. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for researchers studying conditions such as liver disease, vitamin K deficiency, and hepatocellular carcinoma.PIVKA-II, also known as protein induced by vitamin K absence or antagonist-II, is a protein produced in the liver that is elevated in individuals with impaired vitamin K metabolism. Monitoring PIVKA-II levels can provide valuable insights into liver function and vitamin K status, making it a valuable biomarker for diagnostic and research purposes.With easy-to-follow instructions and all necessary components included, the PIVKA-II ELISA Kit is a convenient and efficient tool for detecting and quantifying PIVKA-II levels in human samples. Researchers can confidently rely on this kit for accurate and consistent results in their studies.
Product Name:
Human PIVKA-II ELISA Kit
SKU:
HUES03441
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
87-102
94-107
98-111
Average (%)
94
99
105
1:4
Range (%)
86-100
83-98
87-103
Average (%)
93
90
94
1:8
Range (%)
91-103
79-92
82-95
Average (%)
97
86
89
1:16
Range (%)
89-100
82-98
83-98
Average (%)
94
89
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
92-103
97
Cell Culture Media (n=8)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.26
3.86
0.49
1.15
3.48
Standard deviation
0.03
0.08
0.2
0.03
0.09
0.24
C V (%)
6.54
6.01
5.23
6.3
7.85
6.86
Uniport ID:
P00734
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Human PIVKA-Ⅱ in samples. No significant cross-reactivity or interference between Human PIVKA-Ⅱ and analogues was observed
Storage:
2-8℃
Research Area:
Cardiovascular
Guariglia, M. et al.
Analytical and clinical evaluation of a novel ELISA assay for the measurement of serum PIVKA-II in patients with hepatocellular carcinoma of viral-etiology.
Minerva Biotechnology & Biomolecular Research 2025
