Anti-PIWIL-4 Antibody (CAB13201)

Antibody Name:	PIWIL4 Rabbit Polyclonal Antibody
Antibody SKU:	CAB13201
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 260-460 of human PIWIL4 (NP_689644.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, Mouse testis, Mouse spleen, Rat testis, Rat spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 260-460 of human PIWIL4 (NP_689644.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SADV SYKV LRNE TVLE FMTA LCQR TGLS CFTQ TCEK QLIG LIVL TRYN NRTY SIDD IDWS VKPT HTFQ KRDG TEIT YVDY YKQQ YDIT VSDL NQPM LVSL LKKK RNDN SEAQ LAHL IPEL CFLT GLTD QATS DFQL MKAV AEKT RLSP SGRQ QRLA RLVD NIQR NTNA RFEL ETWG LHFG SQIS LTGR IVPS EKIL MQDH I
Gene ID:	143689
Uniprot:	Q7Z3Z4
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	52kDa/56kDa/96kDa
Observed MW:	105kDa

Synonyms:	PIWIL4, HIWI2, MIWI2
Background:

UniProt Protein Function:	PIWIL4: Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci. Belongs to the argonaute family. Piwi subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Unknown function Chromosomal Location of Human Ortholog: 11q21 Cellular Component: cytoplasm; nucleus Biological Process: regulation of translation; meiotic cell cycle; DNA methylation during gametogenesis; multicellular organismal development; spermatogenesis; RNA-mediated gene silencing; gene expression; cell differentiation
NCBI Summary:	PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar 2008]
UniProt Code:	Q7Z3Z4
NCBI GenInfo Identifier:	116242716
NCBI Gene ID:	143689
NCBI Accession:	Q7Z3Z4.2
UniProt Secondary Accession:	Q7Z3Z4,Q68CZ4, Q8N8G9, Q8N9V8, Q8NEH2, B4DEG5,
UniProt Related Accession:	Q7Z3Z4
Molecular Weight:	852
NCBI Full Name:	Piwi-like protein 4
NCBI Synonym Full Names:	piwi-like RNA-mediated gene silencing 4
NCBI Official Symbol:	PIWIL4
NCBI Official Synonym Symbols:	HIWI2; MIWI2
NCBI Protein Information:	piwi-like protein 4; piwi-like 4
UniProt Protein Name:	Piwi-like protein 4
Protein Family:	Piwi-like protein
UniProt Gene Name:	PIWIL4
UniProt Entry Name:	PIWL4_HUMAN

Western blot analysis of extracts of various cell lines, using PIWIL4 antibody (CAB13201) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Anti-PIWIL-4 Antibody (CAB3144)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)