The POGLUT1 Antibody (CAB17737) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and is highly reactive with human samples, making it suitable for a variety of research applications.POGlut1, also known as O-GlcNAc transferase, plays a crucial role in regulating cellular processes such as transcription, translation, and signaling pathways. Dysregulation of POGlut1 has been implicated in various diseases, including cancer, diabetes, and neurodegenerative disorders. Therefore, investigating the function and expression of POGlut1 is important for understanding its role in disease pathogenesis.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
POGLUT1 Antibody
SKU:
CAB17737
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a protein with both O-glucosyltransferase and O-xylosyltransferase activity which localizes to the lumen of the endoplasmic reticulum. This protein has a carboxy-terminal KTEL motif which is predicted to function as an endoplasmic reticulum retention signal. This gene is an essential regulator of Notch signalling and likely plays a role in cell fate and tissue formation during development. It may also play a role in the pathogenesis of leukemia. Mutations in this gene have been associated with the autosomal dominant genodermatosis Dowling-Degos disease 4. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
56983
RRID
AB_2771735
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using POGLUT1 Rabbit pAb (CAB17737) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
Immunofluorescence analysis of L929 cells using POGLUT1 Rabbit pAb (CAB17737) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
