The DNA Polymerase beta Monoclonal Antibody (CAB2412) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is exceptionally reactive with human samples and has been validated for use in Western blot applications. DNA polymerase beta is crucial for maintaining genomic stability and fidelity, making it a critical player in cellular processes such as DNA damage repair and replication. This antibody binds specifically to the DNA polymerase beta protein, enabling accurate detection and analysis in a variety of cell types.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DNA Polymerase beta Monoclonal Antibody
SKU:
CAB2412
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0752
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
POLB, DNA polymerase beta, DNA Polymerase beta
Positive Sample:
HeLa, A-431, Jurkat, Mouse testis, Mouse spleen, Mouse thymus, Rat testis, Rat spleen
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
38kDa
Observed MW:
42kDa
The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date.
Purification Method
Affinity purification
Gene ID
5423
RRID
AB_2863003
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DNA Polymerase beta Rabbit mAb (CAB2412) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Rat testis using DNA Polymerase beta Rabbit mAb (CAB2412) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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