The Pole Polyclonal Antibody (PACO54226) is a valuable tool for researchers studying Pole, a protein involved in DNA replication and repair processes. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the Pole protein, this antibody allows for the detection and analysis of Pole in a variety of cell types, making it ideal for studies in molecular biology and cancer research.Pole, also known as DNA polymerase epsilon, is essential for maintaining genomic stability and fidelity during cell division.
Dysregulation of Pole has been implicated in various diseases, including cancer and neurological disorders. By studying the function of Pole, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets.Overall, the Pole Polyclonal Antibody (PACO54226) is a valuable tool for researchers interested in exploring the role of Pole in health and disease. Its high specificity and sensitivity make it a reliable choice for a wide range of research applications.
Immunohistochemistry analysis of human breast cancer using PACO54226 at dilution of 1:100.
Immunofluorescent analysis of Hela cells using PACO54226 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Participates in DNA repair and in chromosomal DNA replication.
Synonyms:
DNA polymerase epsilon catalytic subunit A (EC 2.7.7.7) (DNA polymerase II subunit A), POLE, POLE1
UniProt Protein Function:
POLE: Participates in DNA repair and in chromosomal DNA replication. Belongs to the DNA polymerase type-B family.Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; EC 2.7.7.7; DNA replication; DNA repair, damage; TransferaseChromosomal Location of Human Ortholog: 12q24.3Cellular Component: nucleoplasm; epsilon DNA polymerase complex; cytoplasm; plasma membrane; nucleusMolecular Function: DNA binding; zinc ion binding; 4 iron, 4 sulfur cluster binding; nucleotide binding; DNA-directed DNA polymerase activity; chromatin bindingBiological Process: DNA replication initiation; in utero embryonic development; DNA repair; base-excision repair, gap-filling; telomere maintenance via semi-conservative replication; DNA synthesis during DNA repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycleDisease: Colorectal Cancer, Susceptibility To, 12; Facial Dysmorphism, Immunodeficiency, Livedo, And Short Stature
NCBI Summary:
This gene encodes the catalytic subunit of DNA polymerase epsilon. The enzyme is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature. [provided by RefSeq, Sep 2013]
