The POLE2 Antibody (CAB12842) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to Pole2 and is validated for use in Western blot applications. By binding to the Pole2 protein, researchers can detect and analyze its expression in a variety of cell types, making it an essential tool for studies in molecular biology and cancer research.Pole2 is an essential component of the DNA polymerase epsilon complex, which is responsible for DNA replication and repair. Its function is vital for maintaining genomic stability and preventing mutations that can lead to cancer and other genetic disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
POLE2 Antibody
SKU:
CAB12842
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DPE2, POLE2
Positive Sample:
HeLa, Mouse heart
Cellular Localization:
Nucleus.
Calculated MW:
60kDa
Observed MW:
59kDa
DNA polymerase epsilon, which is involved in DNA repair and replication, is composed of a large catalytic subunit and a small accessory subunit. The protein encoded by this gene represents the small subunit (B). Defects in this gene have been linked to colorectal cancer and to combined immunodeficiency.
Purification Method
Affinity purification
Gene ID
5427
RRID
AB_2759683
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using POLE2 Rabbit pAb (CAB12842) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
