The POLRMT Antibody (CAB15605) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
POLRMT Antibody
SKU:
CAB15605
Size:
100μL, 20μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF/ICC
1:50 - 1:200
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
APOLMT, MTRNAP, MTRPOL, COXPD55, h-mtRPOL, POLRMT
Positive Sample:
HeLa, HepG2, 293T
Cellular Localization:
Mitochondrion.
Calculated MW:
139kDa
Observed MW:
139kDa
This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes.
Purification Method
Affinity purification
Gene ID
5442
RRID
AB_2763011
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using POLRMT Rabbit pAb (CAB15605) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of C6 cells using POLRMT Rabbit pAb (CAB15605) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of HepG2 cells, using 3 μg POLRMT antibody (CAB15605). Western blot was performed from the immunoprecipitate using POLRMT antibody (CAB15605) at a dilution of 1:1000.
