The Porcine Cystatin C ELISA Kit offered by Assay Genie is a cutting-edge tool designed for the accurate measurement of cystatin C levels in porcine samples. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it ideal for a variety of research applications. Cystatin C is an important biomarker for renal function and is useful in the assessment of kidney health in pigs. Changes in cystatin C levels can indicate kidney disease or dysfunction, making it a valuable marker for monitoring and diagnosing renal conditions in porcine species.
Whether studying kidney diseases, monitoring renal function in research animals, or developing potential therapies for renal disorders, the Porcine Cystatin C ELISA Kit from Assay Genie is a valuable tool for researchers in the field. Trust in the accuracy and reliability of this kit for your porcine cystatin C testing needs.
Product Name:
Porcine Cystatin C ELISA Kit
SKU:
PRFI00046
Reactivity:
Porcine
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
37.5 pg/mL
Range:
62.5-4000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
86-102%
85-92%
85-101%
EDTA Plasma (n = 5)
83-101%
83-95%
90-99%
Heparin Plasma (n = 5)
82-100%
85-96%
81-97%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-95
90
EDTA Plasma (n = 5)
90-102
97
Heparin Plasma (n = 5)
85-103
95
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
