The Cys-C ELISA Kit is designed for the quantitative detection of Cystatin C (Cys-C) levels in various human biological samples. Cys-C is a major extracellular inhibitor of cysteine proteases and plays a significant role in protein catabolism, cell signaling, and inflammatory response. Its elevated levels are often associated with renal diseases and certain types of cancers. Accurate measurement of Cys-C is crucial for understanding its role in disease mechanisms and for developing targeted therapeutic strategies. Assay Genie's Cys-C ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit delivers robust performance and is easy to use, making it an excellent choice for both research and clinical applications. Trust Assay Genie's Cys-C ELISA Kit for accurate and dependable quantification of this critical biomarker in your studies.
Product Name:
Human Cys-C (Cystatin C) ELISA Kit
SKU:
AEES00039
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
