The Mouse Cys-C (Cystatin C) ELISA Kit is a specialized assay designed for the sensitive and quantitative detection of Cystatin C levels in mouse samples. Cystatin C serves as a valuable biomarker for assessing renal function and is commonly utilized in clinical and research settings to evaluate kidney health and function. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results essential for researchers studying kidney diseases, renal function, and related pathologies. Manufactured under strict quality control standards, the kit provides reliable performance and ease of use, making it an optimal choice for investigative and diagnostic studies on renal health and associated conditions.
Product Name:
Mouse Cys-C (Cystatin C) ELISA Kit
SKU:
AEES00219
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.