The Porcine Defensin Beta 1 (DEFB1) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of DEFB1 levels in porcine serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an excellent tool for researchers studying immune responses in pigs.DEFB1 is an important antimicrobial peptide that plays a critical role in the immune system by defending against pathogens. It is involved in host defense and inflammation, making it an essential biomarker for studying infectious diseases and immune responses in pigs.
With its high sensitivity and accuracy, the Porcine DEFB1 ELISA Kit is ideal for a wide range of research applications, including studying immune responses, infectious diseases, and host-pathogen interactions in pigs. Trust Assay Genie to provide you with a reliable and effective kit for your research needs.
Product Name:
Porcine Defensin Beta 1 (DEFb1) ELISA Kit
Product Code:
PRDL00040
Size:
96 Assays
Target:
DEFb1
Synonyms:
B-DF1, DEFB1, BD1, DEF-B1, DEFB101, HBD1
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
