The Porcine HER2 (Epidermal Growth Factor Receptor 2) ELISA Kit is a powerful tool for the detection and quantification of HER2 levels in porcine samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications. HER2 is a critical protein involved in cell growth and differentiation, and its overexpression has been linked to various types of cancer, including breast cancer and gastric cancer. By measuring HER2 levels in porcine samples, researchers can gain valuable insights into the role of this protein in disease development and progression, potentially leading to new treatment strategies and biomarkers.
Overall, the Porcine HER2 ELISA Kit is a valuable tool for researchers studying cancer, cell signaling pathways, and targeted therapies in porcine models. Its high performance and ease of use make it a versatile solution for laboratories conducting cutting-edge research in the field of molecular biology and oncology.
Serum, Plasma, Cell Culture Supernatant, Cell Lysis Buffer or Tissue Lysis Buffer, and Other Biological Fluid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1
2
3
Serum (n = 5)
86-96%
92-100%
95-101%
EDTA Plasma (n = 5)
87-102%
99-104%
82-99%
Heparin Plasma (n = 5)
83-98%
85-100%
81-95%
Recovery:
Sample
1
2
Serum (n = 5)
93-100
92
EDTA Plasma (n = 5)
85-101
93
Heparin Plasma (n = 5)
86-99
92
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
