Porcine Insulin Like Growth Factor 2 (IGF2) ELISA Kit
The Porcine Insulin-like Growth Factor 2 (IGF2) ELISA Kit is specifically designed for the accurate and precise detection of IGF2 levels in porcine samples, including serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.IGF2 is a critical growth factor involved in regulating cell growth, proliferation, and differentiation. It plays a key role in various physiological processes, including embryonic development, tissue regeneration, and metabolic regulation.
Dysregulation of IGF2 has been linked to a variety of diseases, including cancer, diabetes, and growth disorders, highlighting its importance as a potential biomarker for disease monitoring and therapeutic development.Overall, the Porcine IGF2 ELISA Kit offers researchers a valuable tool for studying the role of IGF2 in porcine physiology and pathology, providing essential insights for advancing our understanding of growth regulation and disease mechanisms in pigs.
Product Name:
Porcine Insulin Like Growth Factor 2 (IGF2) ELISA Kit
Product Code:
PRDL00076
Size:
96 Assays
Target:
IGF2
Synonyms:
INSIGF, IGF-II, Somatomedin A, Preptin, Insulin-like growth factor II Ala-25 Del
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
