Western blot analysis of Mouse brain, using POU4F2 antibody (CAB19331) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The POU4F2 Polyclonal Antibody (CAB19331) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the POU4F2 protein, this antibody enables accurate detection and analysis in various neural cell types, making it an essential component for studies in neuroscience and neurodevelopment.POU4F2, also known as BRN3B, is a transcription factor that regulates the expression of genes involved in neuronal differentiation and survival.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
POU4F2 Polyclonal Antibody
SKU:
CAB19331
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BRN3B, BRN3.2, Brn-3b, POU4F2
Positive Sample:
Mouse brain
Cellular Localization:
Cytoplasm, Nuclear Speck, Nucleoplasm, Nucleus.
Calculated MW:
43kDa
Observed MW:
Refertofigures
The protein encoded by this gene is a member of the POU-domain transcription factor family and may be involved in maintaining visual system neurons in the retina. The level of the encoded protein is also elevated in a majority of breast cancers, resulting in accelerated tumor growth.
Purification Method
Affinity purification
Gene ID
5458
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse brain, using POU4F2 Rabbit pAb (CAB19331) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of U-2 OS cells using POU4F2 Rabbit pAb (CAB19331) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
