Anti-PPIE Antibody (CAB7061)

Antibody Name:	Anti-PPIE Antibody
Antibody SKU:	CAB7061
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-301 of human PPIE (NP_006103.1).

Application:	WB IP
Recommended Dilution:	WB 1:500 - 1:2000 IP 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, THP-1, Jurkat, HL-60, Mouse testis, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-301 of human PPIE (NP_006103.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATT KRVL YVGG LAEE VDDK VLHA AFIP FGDI TDIQ IPLD YETE KHRG FAFV EFEL AEDA AAAI DNMN ESEL FGRT IRVN LAKP MRIK EGSS RPVW SDDD WLKK FSGK TLEE NKEE EGSE PPKA ETQE GEPI AKKA RSNP QVYM DIKI GNKP AGRI QMLL RSDV VPMT AENF RCLC THEK GFGF KGSS FHRI IPQF MCQG GDFT NHNG TGGK SIYG KKFD DENF ILKH TGPG LLSM ANSG PNTN GSQF FLTC DKTD WLDG KHVV FGEV TEGL DVLR QIEA QGSK DGKP KQKV IIAD CGEY V
Gene ID:	10450
Uniprot:	Q9UNP9
Cellular Location:	Nucleus
Calculated MW:	33kDa/34kDa
Observed MW:	33kDa

Synonyms:

PPIE, CYP-33, CYP33

Background:

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified.

UniProt Protein Function:	PPIE: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Combines RNA-binding and PPIase activities. May be involved in muscle- and brain-specific processes. May be involved in pre-mRNA splicing. Belongs to the cyclophilin-type PPIase family. PPIase E subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Spliceosome; Cyclophilin; RNA splicing; EC 5.2.1.8; Isomerase Chromosomal Location of Human Ortholog: 1p32 Cellular Component: nucleoplasm; nucleus Molecular Function:cyclosporin A binding; peptidyl-prolyl cis-trans isomerase activity; protein binding; RNA binding Biological Process: nuclear mRNA splicing, via spliceosome; positive regulation of viral genome replication; regulation of transcription, DNA-dependent; transcription-coupled nucleotide-excision repair
NCBI Summary:	The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified. [provided by RefSeq, Aug 2010]
UniProt Code:	Q9UNP9
NCBI GenInfo Identifier:	13124097
NCBI Gene ID:	10450
NCBI Accession:	Q9UNP9.1
UniProt Secondary Accession:	Q9UNP9,O43634, O43635, Q32Q72, Q3S611, Q5TGA0, Q5TGA2 Q5TGA3, Q9UIZ5, B2R971,
UniProt Related Accession:	Q9UNP9
Molecular Weight:	34,991 Da
NCBI Full Name:	Peptidyl-prolyl cis-trans isomerase E
NCBI Synonym Full Names:	peptidylprolyl isomerase E
NCBI Official Symbol:	PPIE
NCBI Official Synonym Symbols:	CYP33; CYP-33
NCBI Protein Information:	peptidyl-prolyl cis-trans isomerase E
UniProt Protein Name:	Peptidyl-prolyl cis-trans isomerase E
UniProt Synonym Protein Names:	Cyclophilin E; Cyclophilin-33; Rotamase E
UniProt Gene Name:	PPIE
UniProt Entry Name:	PPIE_HUMAN

	Western blot analysis of extracts of various cell lines, using PPIE antibody (CAB7061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug PPIE antibody (CAB7061). Western blot was performed from the immunoprecipitate using PPIE antibody (CAB7061) at a dilition of 1:1000.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)