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Anti-PPIF Antibody (CAB3208)

SKU:
CAB3208
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
€139 - €419
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Description

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Antibody Name:Anti-PPIF Antibody
Antibody SKU:CAB3208
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-207 of human PPIF (NP_005720.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:HepG2, B cells, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-207 of human PPIF (NP_005720.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:CSKG SGDP SSSS SSGN PLVY LDVD ANGK PLGR VVLE LKAD VVPK TAEN FRAL CTGE KGFG YKGS TFHR VIPS FMCQ AGDF TNHN GTGG KSIY GSRF PDEN FTLK HVGP GVLS MANA GPNT NGSQ FFIC TIKT DWLD GKHV VFGH VKEG MDVV KKIE SFGS KSGR TSKK IVIT DCGQ LS
Gene ID:10105
Uniprot:P30405
Cellular Location:Mitochondrion matrix
Calculated MW:16kDa/22kDa
Observed MW:18kDa
Synonyms:PPIF, CYP3, CyP-M, Cyp-D, CypD
Background:The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.
UniProt Protein Function:PPIF: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Belongs to the cyclophilin-type PPIase family.
UniProt Protein Details:

Protein type:EC 5.2.1.8; Isomerase; Mitochondrial

Chromosomal Location of Human Ortholog: 10q22.3

Cellular Component: membrane; mitochondrial matrix; mitochondrial proton-transporting ATP synthase complex

Molecular Function:peptidyl-prolyl cis-trans isomerase activity; protein binding

Biological Process: negative regulation of apoptosis; negative regulation of ATPase activity; regulation of mitochondrial membrane permeability

NCBI Summary:The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008]
UniProt Code:P30405
NCBI GenInfo Identifier:231968
NCBI Gene ID:10105
NCBI Accession:P30405.1
UniProt Secondary Accession:P30405,Q2YDB7, Q5W131,
UniProt Related Accession:P30405
Molecular Weight:16,541 Da
NCBI Full Name:Peptidyl-prolyl cis-trans isomerase F, mitochondrial
NCBI Synonym Full Names:peptidylprolyl isomerase F
NCBI Official Symbol:PPIF  
NCBI Official Synonym Symbols:CYP3; CypD; CyP-M; Cyp-D  
NCBI Protein Information:peptidyl-prolyl cis-trans isomerase F, mitochondrial
UniProt Protein Name:Peptidyl-prolyl cis-trans isomerase F, mitochondrial
UniProt Synonym Protein Names:Cyclophilin D; CyP-D; CypD; Cyclophilin F; Mitochondrial cyclophilin; CyP-M; Rotamase F
Protein Family:Peptidyl-prolyl cis-trans isomerase
UniProt Gene Name:PPIF  
UniProt Entry Name:PPIF_HUMAN
Anti-PPIF Antibody (CAB3208)Western blot analysis of extracts of various cell lines, using PPIF antibody (CAB3208) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

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