Anti-PPP1R9B Antibody (CAB17229)

Antibody Name:	Anti-PPP1R9B Antibody
Antibody SKU:	CAB17229
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human PPP1R9B.

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:

Immunogen:	A synthetic peptide of human PPP1R9B.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	84687
Uniprot:	Q96SB3
Cellular Location:
Calculated MW:	89kDa
Observed MW:	Refer to figures

Synonyms:	PPP1R9B, PPP1R6, PPP1R9, SPINO, Spn
Background:	This gene encodes a scaffold protein that functions as a regulatory subunit of protein phosphatase 1a. Expression of this gene is particularly high in dendritic spines, suggesting that the encoded protein may play a role in receiving signals from the central nervous system. The encoded protein has putative tumor suppressor function and decreased expression has been observed in tumors.

UniProt Protein Function:	Spinophilin: actin-binding regulatory subunit of protein phosphatase 1 (PP1). The actin-binding domain of spinophilin is necessary and sufficient for targeting of spinophilin to dendrites and dendritic spines. May play a role in establishing a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Interacts with p14-ARF. Contains one PDZ/DHR domain.
UniProt Protein Details:	Protein type:Protein phosphatase, regulatory subunit; Adaptor/scaffold Chromosomal Location of Human Ortholog: 17q21.33 Cellular Component: nucleoplasm; cortical actin cytoskeleton; adherens junction; lamellipodium; cytoplasm; dendritic spine; plasma membrane; synapse; protein phosphatase type 1 complex; filopodium Molecular Function:protein binding; protein phosphatase inhibitor activity; actin binding; protein phosphatase 1 binding Biological Process: regulation of synaptic transmission; regulation of protein amino acid phosphorylation; cell migration; regulation of cell growth by extracellular stimulus; filopodium formation; RNA splicing; regulation of exit from mitosis; calcium-mediated signaling; actin filament organization; regulation of cell proliferation; negative regulation of catalytic activity; dendrite development; negative regulation of cell growth; cell cycle arrest
NCBI Summary:	This gene encodes a scaffold protein that functions as a regulatory subunit of protein phosphatase 1a. Expression of this gene is particularly high in dendritic spines, suggesting that the encoded protein may play a role in receiving signals from the central nervous system. The encoded protein has putative tumor suppressor function and decreased expression has been observed in tumors. [provided by RefSeq, Feb 2014]
UniProt Code:	Q96SB3
NCBI GenInfo Identifier:	90101416
NCBI Gene ID:	84687
NCBI Accession:	Q96SB3.2
UniProt Secondary Accession:	Q96SB3,Q8TCR9,
UniProt Related Accession:	Q96SB3
Molecular Weight:	815
NCBI Full Name:	Neurabin-2
NCBI Synonym Full Names:	protein phosphatase 1, regulatory subunit 9B
NCBI Official Symbol:	PPP1R9B
NCBI Official Synonym Symbols:	Spn; SPINO; PPP1R6; PPP1R9
NCBI Protein Information:	neurabin-2; neurabin-II; protein phosphatase 1, regulatory (inhibitor) subunit 9B; protein phosphatase 1, regulatory subunit 9B, spinophilin
UniProt Protein Name:	Neurabin-2
UniProt Synonym Protein Names:	Neurabin-II; Protein phosphatase 1 regulatory subunit 9B; Spinophilin
UniProt Gene Name:	PPP1R9B
UniProt Entry Name:	NEB2_HUMAN

Western blot analysis of extracts of various cell lines, using PPP1R9B antibody (CAB17229) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.

Antibodies	ELISA Kits
Anti-PPP1R9B Antibody (CAB17230)	Human Neurabin-2 / PPP1R9B ELISA Kit
PPP1R9B Antibody (PACO11428)	Human Neurabin-2 (PPP1R9B) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)