Anti-PRDM14 Antibody (CAB5543)

Antibody Name:	Anti-PRDM14 Antibody
Antibody SKU:	CAB5543
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP ChIP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-571 of human PRDM14 (AAH52311.1).

Application:	WB IHC IF IP ChIP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:20 - 1:100 ChIP 1:20 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-571 of human PRDM14 (AAH52311.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MALP RPSE AVPQ DKVC YPPE SSPQ NLAA YYTP FPSY GHYR NSLA TVEE DFQP FRQL EAAA SAAP AMPP FPFR MAPP LLSP GLGL QREP LYDL PWYS KLPP WYPI PHVP REVP PFLS SSHE YAGA SSED LGHQ IIGG DNES GPCC GPDT LIPP PPAD ASLL PEGL RTSQ LLPC SPSK QSED GPKP SNQE GKSP ARFQ FTEE DLHF VLYG VTPS LEHP ASLH HAIS GLLV PPDS SGSD SLPQ TLDE DSLQ LPEG LCLM QTVF GEVP HFGV FCSS FIAK GVRF GPFQ GKVV NASE VKTY GDNS VMWE IFED GHLS HFID GKGG TGNW MSYV NCAR FPKE QNLV AVQC QGHI FYES CKEI HQNQ ELLV WYGD CYEK FLDI PVSL QVTE PGKQ PSGP SEES AEGY RCER CGKV FTYK YYRD KHLK YTPC VDKG DRKF PCSL CKRS FEKR DRLR IHIL HVHE KHRP HKCS TCGK CFSQ SSSL NKHM RVHS GDRP YQCV YCTK RFTA SSIL RTHI RQHS GEKP FKCK YCGK SFAS HAAH DSHV RRSH KEDD GCSC SICG KIFS DQET FYSH MKFH EDY
Gene ID:	63978
Uniprot:	Q9GZV8
Cellular Location:	Nucleus
Calculated MW:	64kDa
Observed MW:	64kDa

Synonyms:	PRDM14, PFM11
Background:	This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer.

UniProt Protein Function:	PRDM14: a probable protein lysine methyltransferase that works in concert with the core human embryonic stem cell (ESC) regulators to control pluripotency associated genes. Binds to silenced genes in human ESCs. Its binding profile is enriched for the repressive tri-methylation of histone H3 lysine 27 (H3K27me3) modification. Interacts directly with the polycomb repressive complex 2 (PRC2). PRC2 is detected at PRDM14-bound loci in human ESCs. A probable transcriptional regulator of the krueppel C2H2-type zinc-finger protein family. Has both positive and negative roles on transcription. Required for the maintenance of emryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming. Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.
UniProt Protein Details:	Protein type:Methyltransferase, protein arginine, predicted; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 8q13.3 Cellular Component: nucleoplasm Molecular Function:methyltransferase activity; protein binding; DNA binding; metal ion binding Biological Process: fertilization; regulation of transcription, DNA-dependent; transcription, DNA-dependent; homeostasis of number of cells within a tissue; negative regulation of fibroblast growth factor receptor signaling pathway; somatic stem cell maintenance; inner cell mass cell fate commitment; cell morphogenesis; germ-line stem cell maintenance; cell fate specification; regulation of gene expression, epigenetic; germ cell development
NCBI Summary:	This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer. [provided by RefSeq, Dec 2011]
UniProt Code:	Q9GZV8
NCBI GenInfo Identifier:	17368925
NCBI Gene ID:	63978
NCBI Accession:	Q9GZV8.1
UniProt Secondary Accession:	Q9GZV8,Q86UX9,
UniProt Related Accession:	Q9GZV8
Molecular Weight:	571
NCBI Full Name:	PR domain zinc finger protein 14
NCBI Synonym Full Names:	PR domain containing 14
NCBI Official Symbol:	PRDM14
NCBI Official Synonym Symbols:	PFM11
NCBI Protein Information:	PR domain zinc finger protein 14; PR domain-containing 14; PR domain-containing protein 14
UniProt Protein Name:	PR domain zinc finger protein 14
UniProt Synonym Protein Names:	PR domain-containing protein 14
Protein Family:	PR domain zinc finger protein
UniProt Gene Name:	PRDM14
UniProt Entry Name:	PRD14_HUMAN

	Western blot analysis of extracts of various cell lines, using PRDM14 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
	Western blot analysis of extracts of NCI-H125 cells, using PRDM14 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat testis using PRDM14 Rabbit pAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Chromatin immunoprecipitation analysis of RUNX1 gene from 293 cell line, using PRDM14 antibody and rabbit IgG. P1, P2, P3 and P4 were four probes located on RUNX1 gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Antibodies

Anti-PRDM14 Antibody (CAB13658)

Anti-PRDM14 Antibody (CAB17203)

Anti-PRDM14 Antibody (CAB17204)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)