Anti-PSEN2 Antibody (CAB7719)

Antibody Name:	Anti-PSEN2 Antibody
Antibody SKU:	CAB7719
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human PSEN2.

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, Mouse brain, Rat brain

Immunogen:	Recombinant protein of human PSEN2.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	5664
Uniprot:	P49810
Cellular Location:	Endoplasmic reticulum membrane, Golgi apparatus membrane, Multi-pass membrane protein
Calculated MW:	46kDa/50kDa
Observed MW:	21KDa/35KDa/50KDa

Synonyms:

PSEN2, AD3L, AD4, CMD1V, PS2, STM2

Background:

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified.

UniProt Protein Function:	PSEN2: probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). An integral membrane protein of the Golgi and endoplasmic reticulum. Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins. Two alternative splice isoforms have been identified.
UniProt Protein Details:	Protein type:EC 3.4.23.-; Membrane protein, integral; Membrane protein, multi-pass; Cell surface; Protease Chromosomal Location of Human Ortholog: 1q42.13 Cellular Component: Golgi apparatus; centrosome; cell surface; integral to plasma membrane; lysosomal membrane; nuclear inner membrane; cytosol; lipid raft; ciliary rootlet; kinetochore; cell soma; membrane; perinuclear region of cytoplasm; apical plasma membrane; mitochondrial inner membrane; dendritic shaft; neuromuscular junction; endoplasmic reticulum membrane; protein complex; endoplasmic reticulum; cell cortex; Z disc; Golgi membrane; growth cone; axon; plasma membrane Molecular Function:protein binding; endopeptidase activity; aspartic-type endopeptidase activity Biological Process: positive regulation of catalytic activity; axon guidance; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; protein maturation; regulation of synaptic plasticity; positive regulation of coagulation; cell fate specification; T cell receptor signaling pathway; T cell activation during immune response; protein transport; amyloid precursor protein catabolic process; hair follicle development; negative regulation of protein amino acid phosphorylation; beta-amyloid metabolic process; calcium ion transport; forebrain development; ephrin receptor signaling pathway; brain morphogenesis; negative regulation of protein binding; dorsoventral neural tube patterning; embryonic limb morphogenesis; cardiac muscle contraction; Notch signaling pathway; somitogenesis; membrane protein intracellular domain proteolysis; thymus development; regulation of epidermal growth factor receptor activity; membrane protein ectodomain proteolysis; Notch receptor processing; memory; endoplasmic reticulum calcium ion homeostasis; myeloid leukocyte differentiation; response to hypoxia; hemopoietic progenitor cell differentiation; protein processing; negative regulation of protein complex assembly; alveolus development; negative regulation of apoptosis Disease: Alzheimer Disease 4; Cardiomyopathy, Dilated, 1v
NCBI Summary:	Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	P49810
NCBI GenInfo Identifier:	1709858
NCBI Gene ID:	5664
NCBI Accession:	P49810.1
UniProt Secondary Accession:	P49810,Q96P32, A8K8D4, B1AP21,
UniProt Related Accession:	P49810
Molecular Weight:	50,011 Da
NCBI Full Name:	Presenilin-2
NCBI Synonym Full Names:	presenilin 2
NCBI Official Symbol:	PSEN2
NCBI Official Synonym Symbols:	AD4; PS2; AD3L; STM2; CMD1V
NCBI Protein Information:	presenilin-2; AD5; E5-1; PS-2; AD3LP; STM-2; Alzheimer disease 4
UniProt Protein Name:	Presenilin-2
UniProt Synonym Protein Names:	AD3LP; AD5; E5-1; STM-2Cleaved into the following 2 chains:Presenilin-2 NTF subunit; Presenilin-2 CTF subunit
Protein Family:	Presenilin
UniProt Gene Name:	PSEN2
UniProt Entry Name:	PSN2_HUMAN

	Western blot analysis of extracts of various cell lines, using PSEN2 antibody (CAB7719) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat heart using PSEN2 antibody (CAB7719) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney using PSEN2 antibody (CAB7719) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using PSEN2 Rabbit pAb (CAB7719) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Phospho-PSEN2 (S330) Antibody (PACO04779)	Human PSEN2 / Presenilin-2 ELISA Kit
	Human Presenilin-2 (PSEN2) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)