Anti-PRKAB2 Antibody (CAB6952)

Antibody Name:	Anti-PRKAB2 Antibody
Antibody SKU:	CAB6952
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-272 of human PRKAB2 (NP_005390.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, Mouse heart, Mouse brain, Rat brain, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-272 of human PRKAB2 (NP_005390.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGNT TSDR VSGE RHGA KAAR SEGA GGHA PGKE HKIM VGST DDPS VFSL PDSK LPGD KEFV SWQQ DLED SVKP TQQA RPTV IRWS EGGK EVFI SGSF NNWS TKIP LIKS HNDF VAIL DLPE GEHQ YKFF VDGQ WVHD PSEP VVTS QLGT INNL IHVK KSDF EVFD ALKL DSME SSET SCRD LSSS PPGP YGQE MYAF RSEE RFKS PPIL PPHL LQVI LNKD TNIS CDPA LLPE PNHV MLNH LYAL SIKD SVMV LSAT HRYK KKYV TTLL YKPI
Gene ID:	5565
Uniprot:	O43741
Cellular Location:
Calculated MW:	21kDa/30kDa
Observed MW:	35kDa

Synonyms:

PRKAB2

Background:

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Multiple alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	AMPKB2: a non-catalytic subunit of AMPK, a conserved kinase of the CAMKL family. AMPK is an energy-sensing protein that plays a key role in regulating cellular energy homeostasis. Environmental stress, such as heat shock, nutrient deprivation, hypoxia and ischemia, indirectly activate AMPK by the depletion of cellular ATP and the concomitant rise of ADP and AMP levels. Allosteric activation is achieved primarily by rising ADP levels, and not solely by AMP levels as previously thought. Activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Beta subunits act as scaffolds on which the AMPK complex assembles, via its C-terminus that bridges alpha and gamma subunits. AMPK-beta1 or -beta2 subunits are required for assembling of AMPK heterotrimers and are important for regulating enzyme activity and cellular localization. AMPK beta1beta2 null mouse muscles reveal an essential role for AMPK in maintaining mitochondrial content and glucose uptake during exercise. Phosphorylation by ULK1 and ULK2 inhibits AMPK activity.
UniProt Protein Details:	Protein type:Protein kinase, regulatory subunit; Autophagy Chromosomal Location of Human Ortholog: 1q21.1 Cellular Component: nucleoplasm; cytosol; AMP-activated protein kinase complex Molecular Function:AMP-activated protein kinase activity; identical protein binding; protein binding Biological Process: mitochondrion organization and biogenesis; regulation of fatty acid biosynthetic process; organelle organization and biogenesis; insulin receptor signaling pathway; energy reserve metabolic process; carnitine shuttle; cellular lipid metabolic process; cell cycle arrest; regulation of protein kinase activity; signal transduction; protein amino acid phosphorylation; fatty acid biosynthetic process
NCBI Summary:	The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2013]
UniProt Code:	O43741
NCBI GenInfo Identifier:	3912957
NCBI Gene ID:	5565
NCBI Accession:	O43741.1
UniProt Related Accession:	O43741
Molecular Weight:	21kDa; 30kDa
NCBI Full Name:	5'-AMP-activated protein kinase subunit beta-2
NCBI Synonym Full Names:	protein kinase AMP-activated non-catalytic subunit beta 2
NCBI Official Symbol:	PRKAB2
NCBI Protein Information:	5'-AMP-activated protein kinase subunit beta-2
UniProt Protein Name:	5'-AMP-activated protein kinase subunit beta-2
Protein Family:	5'-AMP-activated protein kinase
UniProt Gene Name:	PRKAB2
UniProt Entry Name:	AAKB2_HUMAN

	Western blot analysis of extracts of various cell lines, using PRKAB2 antibody (CAB6952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using PRKAB2 antibody (CAB6952) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using PRKAB2 Polyclonal Antibody (CAB6952) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using PRKAB2 Polyclonal Antibody (CAB6952) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using PRKAB2 Polyclonal Antibody (CAB6952) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-PRKAB2 Antibody (CAB13341)

PRKAB2 Antibody (PACO03306)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)