Anti-PRKD3 Antibody (CAB7084)

Antibody Name:	Anti-PRKD3 Antibody
Antibody SKU:	CAB7084
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human PRKD3 (NP_005804.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	SW480, NCI-H460, HeLa, Mouse ovary

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human PRKD3 (NP_005804.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSAN NSPP SAQK SVLP TAIP AVLP AASP CSSP KTGL SARL SNGS FSAP SLTN SRGS VHTV SFLL QIGL TRES VTIE AQEL SLSA VKDL VCSI VYQK FPEC
Gene ID:	23683
Uniprot:	O94806
Cellular Location:	Cytoplasm, Membrane
Calculated MW:	68kDa/100kDa
Observed MW:	70kDa

Synonyms:

PRKD3, EPK2, PKC-NU, PKD3, PRKCN, nPKC-NU

Background:

This gene belongs to the multigene protein kinase D family of serine/threonine kinases, which bind diacylglycerol and phorbol esters. Members of this family are characterized by an N-terminal regulatory domain comprised of a tandem repeat of cysteine-rich zinc-finger motifs and a pleckstrin domain. The C-terminal region contains the catalytic domain and is distantly related to calcium-regulated kinases. Catalytic activity of this enzyme promotes its nuclear localization. This protein has been implicated in a variety of functions including negative regulation of human airway epithelial barrier formation, growth regulation of breast and prostate cancer cells, and vesicle trafficking.

UniProt Protein Function:	PRKD3: a CAMK kinase of the PKD family. An important component of signaling pathways downstream from novel PKC enzymes after B-cell receptor engagement. Novel isoforms regulate human keratinocyte differentiation
UniProt Protein Details:	Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.13; CAMK group; PKD family Chromosomal Location of Human Ortholog: 2p21 Cellular Component: cytoplasm; nucleoplasm Molecular Function:kinase activity; protein binding Biological Process: protein kinase C activation
NCBI Summary:	This gene belongs to the multigene protein kinase D family of serine/threonine kinases, which bind diacylglycerol and phorbol esters. Members of this family are characterized by an N-terminal regulatory domain comprised of a tandem repeat of cysteine-rich zinc-finger motifs and a pleckstrin domain. The C-terminal region contains the catalytic domain and is distantly related to calcium-regulated kinases. Catalytic activity of this enzyme promotes its nuclear localization. This protein has been implicated in a variety of functions including negative regulation of human airway epithelial barrier formation, growth regulation of breast and prostate cancer cells, and vesicle trafficking. [provided by RefSeq, Jan 2015]
UniProt Code:	O94806
NCBI GenInfo Identifier:	12230239
NCBI Gene ID:	23683
NCBI Accession:	O94806.1
UniProt Secondary Accession:	O94806,Q53TR7, Q8NEL8, D6W587,
UniProt Related Accession:	O94806
Molecular Weight:	68,123 Da
NCBI Full Name:	Serine/threonine-protein kinase D3
NCBI Synonym Full Names:	protein kinase D3
NCBI Official Symbol:	PRKD3
NCBI Official Synonym Symbols:	EPK2; PKD3; PRKCN; PKC-NU; nPKC-NU
NCBI Protein Information:	serine/threonine-protein kinase D3
UniProt Protein Name:	Serine/threonine-protein kinase D3
UniProt Synonym Protein Names:	Protein kinase C nu type; Protein kinase EPK2; nPKC-nu
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	PRKD3
UniProt Entry Name:	KPCD3_HUMAN

	Western blot analysis of extracts of various cell lines, using PRKD3 antibody (CAB7084) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of A549 cells using PRKD3 antibody (CAB7084). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)