The PRKDC Antibody (CAB7716) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity for human samples and is validated for use in Western blot applications. By targeting the PRKDC protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in genetics, oncology, and molecular biology.PRKDC, also known as DNA-dependent protein kinase catalytic subunit, plays a crucial role in responding to DNA damage and maintaining genomic integrity. Dysregulation of PRKDC has been implicated in various diseases, including cancer and genetic disorders.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
PRKDC Antibody
SKU:
CAB7716
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.
Purification Method
Affinity purification
Gene ID
5591
RRID
AB_2771830
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DNA-PKcs Rabbit pAb (CAB7716) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human well-differentiated squamous skin carcinoma using DNA-PKcs Rabbit pAb (CAB7716) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse liver using DNA-PKcs Rabbit pAb (CAB7716) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of U-2OS cells using DNA-PKcs Rabbit pAb (CAB7716) at dilution of 1:100. Blue: DAPI for nuclear staining.
