The PRMT5 Antibody (CAB2290) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the PRMT5 protein, researchers can detect and analyze its expression in various cell types, making it an essential reagent for studies in epigenetics, gene regulation, and cancer research.PRMT5, also known as protein arginine methyltransferase 5, is a key enzyme involved in the methylation of arginine residues in histones and other proteins, thereby impacting various cellular functions such as transcriptional regulation, RNA processing, and cell cycle progression.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PRMT5 Antibody
SKU:
CAB2290
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HSL7, JBP1, SKB1, IBP72, SKB1Hs, HRMT1L5, PRMT5
Positive Sample:
HepG2, Jurkat, Raji
Cellular Localization:
Cytoplasm, Golgi Apparatus, Nucleus.
Calculated MW:
73kDa
Observed MW:
73kDa
This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
10419
RRID
AB_2764274
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PRMT5 Rabbit pAb (CAB2290). Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
