Anti-NPY Antibody (CAB3178)

Antibody Name:	Anti-NPY Antibody
Antibody SKU:	CAB3178
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-97 of human NPY (NP_000896.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-97 of human NPY (NP_000896.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLGN KRLG LSGL TLAL SLLV CLGA LAEA YPSK PDNP GEDA PAED MARY YSAL RHYI NLIT RQRY GKRS SPET LISD LLMR ESTE NVPR TRLE DPAM W
Gene ID:	4852
Uniprot:	P01303
Cellular Location:	Secreted
Calculated MW:	10kDa
Observed MW:	11KDa

Synonyms:

NPY, PYY4

Background:

This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases. The protein also exhibits antimicrobial activity against bacteria and fungi.

UniProt Protein Function:	NPY: NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone. Belongs to the NPY family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide; Hormone Chromosomal Location of Human Ortholog: 7p15.1 Cellular Component: Golgi apparatus; extracellular space; cell; extracellular region Molecular Function:G-protein coupled receptor activity; neuropeptide hormone activity; neuropeptide Y receptor binding; calcium channel regulator activity; receptor binding Biological Process: blood circulation; behavior; adult feeding behavior; G-protein signaling, coupled to cyclic nucleotide second messenger; synaptic transmission; cell proliferation; neuropeptide signaling pathway; regulation of blood pressure; calcium ion transport; positive regulation of appetite; regulation of appetite; digestion; feeding behavior; cerebral cortex development; cell motility; neurite development; central nervous system neuron development
NCBI Summary:	This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases. The protein also exhibits antimicrobial activity against bacteria and fungi. [provided by RefSeq, Oct 2014]
UniProt Code:	P01303
NCBI GenInfo Identifier:	128117
NCBI Gene ID:	4852
NCBI Accession:	P01303.1
UniProt Related Accession:	P01303
Molecular Weight:	10 kDa
NCBI Full Name:	Pro-neuropeptide Y
NCBI Synonym Full Names:	neuropeptide Y
NCBI Official Symbol:	NPY
NCBI Official Synonym Symbols:	PYY4
NCBI Protein Information:	pro-neuropeptide Y; prepro-neuropeptide Y
UniProt Protein Name:	Pro-neuropeptide Y
UniProt Synonym Protein Names:	Neuropeptide tyrosine; NPY
Protein Family:	Neuropeptide
UniProt Gene Name:	NPY
UniProt Entry Name:	NPY_HUMAN

	Western blot analysis of extracts of SH-SY5Y cells, using NPY antibody (CAB3178) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded mouse spinal cord using NPY Rabbit pAb (CAB3178) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-NPY Antibody (CAB15076)	Human Neuropeptide Y / NPY ELISA Kit
NPY Antibody (PACO04456)	Human Pro-neuropeptide Y (NPY) ELISA Kit
NPY Antibody (PACO10914)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)