Anti-Protein Wnt-5b Antibody (CAB8313)

Antibody Name:	WNT5B Rabbit Polyclonal Antibody
Antibody SKU:	CAB8313
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-359 of human WNT5B (NP_116031.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, 293T, SH-SY5Y, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-359 of human WNT5B (NP_116031.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VSAA GVVN AISR ACRE GELS TCGC SRTA RPKD LPRD WLWG GCGD NVEY GYRF AKEF VDAR EREK NFAK GSEE QGRV LMNL QNNE AGRR AVYK MADV ACKC HGVS GSCS LKTC WLQL AEFR KVGD RLKE KYDS AAAM RVTR KGRL ELVN SRFT QPTP EDLV YVDP SPDY CLRN ESTG SLGT QGRL CNKT SEGM DGCE LMCC GRGY NQFK SVQV ERCH CKFH WCCF VRCK KCTE IVDQ YICK
Gene ID:	81029
Uniprot:	Q9H1J7
Cellular Location:	Secreted, extracellular matrix, extracellular space
Calculated MW:	40kDa
Observed MW:	50kDa

Synonyms:

WNT5B

Background:

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants.

UniProt Protein Function:	WNT5B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Interacts with PORCN. Belongs to the Wnt family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 12p13.3 Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane Molecular Function:frizzled binding; receptor binding Biological Process: cell fate commitment; chondrocyte differentiation; neuron differentiation; positive regulation of cell migration; Wnt receptor signaling pathway
NCBI Summary:	The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9H1J7
NCBI GenInfo Identifier:	20532427
NCBI Gene ID:	81029
NCBI Accession:	Q9H1J7.2
UniProt Secondary Accession:	Q9H1J7,Q96S49, Q9BV04, A8K315, D3DUP9,
UniProt Related Accession:	Q9H1J7
Molecular Weight:	40,323 Da
NCBI Full Name:	Protein Wnt-5b
NCBI Synonym Full Names:	Wnt family member 5B
NCBI Official Symbol:	WNT5B
NCBI Protein Information:	protein Wnt-5b
UniProt Protein Name:	Protein Wnt-5b
UniProt Gene Name:	WNT5B
UniProt Entry Name:	WNT5B_HUMAN

	Western blot analysis of extracts of various cell lines, using WNT5B antibody (CAB8313) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded rat lung using WNT5B antibody (CAB8313) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat bone marrow using WNT5B antibody (CAB8313) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat heart using WNT5B antibody (CAB8313) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using WNT5B antibody (CAB8313) at dilution of 1:100 (40x lens).

ELISA Kits

Human Wnt-5b ELISA Kit

Human Protein Wnt-5b (WNT5B) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)