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Anti-PRPF4 Antibody (CAB6052)

SKU:
CAB6052
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 2 Anti-PRPF4 Antibody CAB6052
  • Epigenetics and Nuclear Signaling Antibodies 2 Anti-PRPF4 Antibody CAB6052
  • Epigenetics and Nuclear Signaling Antibodies 2 Anti-PRPF4 Antibody CAB6052
  • Epigenetics and Nuclear Signaling Antibodies 2 Anti-PRPF4 Antibody CAB6052
  • Epigenetics and Nuclear Signaling Antibodies 2 Anti-PRPF4 Antibody CAB6052
€139 - €419
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Description

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Antibody Name:Anti-PRPF4 Antibody
Antibody SKU:CAB6052
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PRPF4 (NP_004688.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, SKOV3, SW480, DU145, MCF7, Mouse spleen, Mouse thymus, Mouse testis, Rat spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PRPF4 (NP_004688.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MASS RASS TQAT KTKA PDDL VAPV VKKP HIYY GSLE EKER ERLA KGES GILG KDGL KAGI EAGN INIT SGEV FEIE EHIS ERQA EVLA EFER RKRA RQIN VSTD DSEV KACL RALG EPIT LFGE GPAE RRER LRNI LSVV GTDA LKKT KKDD EKSK KSKE EYQQ TWYH EGPN SLKV ARLW IANY SLPR AMKR LEEA RLHK EIPE TTRT SQMQ ELHK SLRS LNNF CSQI GDDR PISY CHFS PNSK MLAT ACWS GLCK LWSV
Gene ID:9128
Uniprot:O43172
Cellular Location:Nucleus speckle
Calculated MW:58kDa
Observed MW:58kDa
Synonyms:PRPF4, HPRP4, HPRP4P, PRP4, Prp4p, RP70, SNRNP60
Background:The protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:PRPF4: Involved in pre-mRNA splicing. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Spliceosome; RNA processing; RNA splicing

Chromosomal Location of Human Ortholog: 9q31-q33

Cellular Component: nucleoplasm; Cajal body; spliceosome; U4/U6 x U5 tri-snRNP complex; nuclear speck; nucleus

Molecular Function:U4 snRNA binding; protein binding; U6 snRNA binding

Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; RNA splicing, via transesterification reactions; RNA splicing; gene expression

Disease: Retinitis Pigmentosa 70

NCBI Summary:The protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
UniProt Code:O43172
NCBI GenInfo Identifier:20514971
NCBI Gene ID:9128
NCBI Accession:O43172.2
UniProt Secondary Accession:O43172,O43445, O43864, Q5T1M8, Q96DG2, Q96IK4,
UniProt Related Accession:O43172
Molecular Weight:522
NCBI Full Name:U4/U6 small nuclear ribonucleoprotein Prp4
NCBI Synonym Full Names:pre-mRNA processing factor 4
NCBI Official Symbol:PRPF4  
NCBI Official Synonym Symbols:PRP4; RP70; HPRP4; Prp4p; HPRP4P; SNRNP60  
NCBI Protein Information:U4/U6 small nuclear ribonucleoprotein Prp4; PRP4 homolog; WD splicing factor Prp4; U4/U6 snRNP 60 kDa protein; PRP4/STK/WD splicing factor; PRP4 pre-mRNA processing factor 4 homolog
UniProt Protein Name:U4/U6 small nuclear ribonucleoprotein Prp4
UniProt Synonym Protein Names:PRP4 homolog; hPrp4; U4/U6 snRNP 60 kDa protein; WD splicing factor Prp4
UniProt Gene Name:PRPF4  
UniProt Entry Name:PRP4_HUMAN
Anti-PRPF4 Antibody (CAB6052)Western blot analysis of extracts of various cell lines, using PRPF4 antibody (CAB6052) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-PRPF4 Antibody (CAB6052)Immunohistochemistry of paraffin-embedded rat lung using PRPF4 Rabbit pAb (CAB6052) at dilution of 1:200 (40x lens).
Anti-PRPF4 Antibody (CAB6052)Immunohistochemistry of paraffin-embedded rat spleen using PRPF4 Rabbit pAb (CAB6052) at dilution of 1:200 (40x lens).
Anti-PRPF4 Antibody (CAB6052)Immunohistochemistry of paraffin-embedded human brain using PRPF4 Rabbit pAb (CAB6052) at dilution of 1:200 (40x lens).
Anti-PRPF4 Antibody (CAB6052)Immunohistochemistry of paraffin-embedded human breast cancer using PRPF4 Rabbit pAb (CAB6052) at dilution of 1:200 (40x lens).

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