Anti-PSMA4 Antibody (CAB13535)

Antibody Name:	Anti-PSMA4 Antibody
Antibody SKU:	CAB13535
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat, Monkey
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PSMA4 (NP_001096137.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat, Monkey
Positive Samples:	293T, U-251MG, HeLa, COS-7, PC-12, Jurkat, Mouse liver, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human PSMA4 (NP_001096137.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSRR YDSR TTIF SPEG RLYQ VEYA MEAI GHAG TCLG ILAN DGVL LAAE RRNI HKLL DEVF FSEK IYKL NEDM ACSV AGIT SDAN VLTN ELRL IAQR YLLQ YQEP IPCE QLVT ALCD IKQA YTQF GGKR PFGV SLLY IGWD KHYG FQLY QSDP SGNY GGWK ATCI GNNS AAAV SMLK QDYK EGEM TLKS ALAL AIKV LNKT MDVS KLSA EKVE IATL TREN GKTV IRVL KQKE VEQL IKKH EEEE AKAE REKK EKEQ KEKD K
Gene ID:	5685
Uniprot:	P25789
Cellular Location:	Cytoplasm, Nucleus, P-body
Calculated MW:	21kDa/29kDa
Observed MW:	29kDa

Synonyms:

PSMA4, HC9, HsT17706, PSC9

Background:

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PSMA4: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Belongs to the peptidase T1A family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.4.25.1; Proteasome complex; Protease Chromosomal Location of Human Ortholog: 15q25.1 Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; intracellular membrane-bound organelle; cytoplasm; plasma membrane; nucleus; cytosol Molecular Function:threonine endopeptidase activity; protein binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
NCBI Summary:	This gene encodes a core alpha subunit of the 20S proteosome, which is a highly ordered ring-shaped structure composed of four rings of 28 non-identical subunits. Proteasomes cleave peptides in an ATP- and ubiquitin-dependent manner. [provided by RefSeq, Aug 2016]
UniProt Code:	P25789
NCBI GenInfo Identifier:	130861
NCBI Gene ID:	5685
NCBI Accession:	P25789.1
UniProt Related Accession:	P25789
Molecular Weight:	29 kDa
NCBI Full Name:	Proteasome subunit alpha type-4
NCBI Synonym Full Names:	proteasome 20S subunit alpha 4
NCBI Official Symbol:	PSMA4
NCBI Official Synonym Symbols:	HC9; PSC9; HsT17706
NCBI Protein Information:	proteasome subunit alpha type-4
UniProt Protein Name:	Proteasome subunit alpha type-4
UniProt Synonym Protein Names:	Macropain subunit C9; Multicatalytic endopeptidase complex subunit C9; Proteasome component C9; Proteasome subunit L
Protein Family:	Proteasome
UniProt Gene Name:	PSMA4
UniProt Entry Name:	PSA4_HUMAN

	Western blot analysis of extracts of various cell lines, using PSMA4 antibody (CAB13535) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human colon using PSMA4 antibody (CAB13535) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using PSMA4 antibody (CAB13535). Blue: DAPI for nuclear staining.

Antibodies

Anti-PSMA4 Antibody (CAB2511)

PSMA4 Antibody (PACO11525)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)