Anti-PSMA5 Antibody (CAB14053)

Antibody Name:	Anti-PSMA5 Antibody
Antibody SKU:	CAB14053
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-241 of human PSMA5 (NP_002781.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, 293T, HepG2, U-251MG, BxPC-3, Mouse liver, Mouse thymus, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-241 of human PSMA5 (NP_002781.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MFLT RSEY DRGV NTFS PEGR LFQV EYAI EAIK LGST AIGI QTSE GVCL AVEK RITS PLME PSSI EKIV EIDA HIGC AMSG LIAD AKTL IDKA RVET QNHW FTYN ETMT VESV TQAV SNLA LQFG EEDA DPGA MSRP FGVA LLFG GVDE KGPQ LFHM DPSG TFVQ CDAR AIGS ASEG AQSS LQEV YHKS MTLK EAIK SSLI ILKQ VMEE KLNA TNIE LATV QPGQ NFHM FTKE ELEE VIKD I
Gene ID:	5686
Uniprot:	P28066
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	20kDa/26kDa
Observed MW:	26kDa

Synonyms:

PSMA5, PSC5, ZETA

Background:

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene.

UniProt Protein Function:	PSMA5: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. PSMA5 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly. Up-regulated in colon cancer cell lines. Up-regulated in fetal Down syndrome (DS) brain. May be the target of the transcriptional activator NFE2L2. Expressed in fetal brain. Belongs to the peptidase T1A family.
UniProt Protein Details:	Protein type:EC 3.4.25.1; Proteasome complex; Protease Chromosomal Location of Human Ortholog: 1p13 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; proteasome complex; proteasome core complex Molecular Function:protein binding Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway
NCBI Summary:	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
UniProt Code:	P28066
NCBI GenInfo Identifier:	38258905
NCBI Gene ID:	5686
NCBI Accession:	P28066.3
UniProt Secondary Accession:	P28066,Q3T1C1, Q6IBF7, B2R8F6, B4E2V4,
UniProt Related Accession:	P28066
Molecular Weight:	20,035 Da
NCBI Full Name:	Proteasome subunit alpha type-5
NCBI Synonym Full Names:	proteasome subunit alpha 5
NCBI Official Symbol:	PSMA5
NCBI Official Synonym Symbols:	PSC5; ZETA
NCBI Protein Information:	proteasome subunit alpha type-5
UniProt Protein Name:	Proteasome subunit alpha type-5
UniProt Synonym Protein Names:	Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; Proteasome zeta chain
UniProt Gene Name:	PSMA5
UniProt Entry Name:	PSA5_HUMAN

	Western blot analysis of extracts of various cell lines, using PSMA5 antibody (CAB14053) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat lung using PSMA5 Antibody (CAB14053) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using PSMA5 Antibody (CAB14053) at dilution of 1:100 (40x lens).

Antibodies

Anti-PSMA5 Antibody (CAB5752)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)