Anti-PSMB10 Antibody (CAB5452)

Antibody Name:	Anti-PSMB10 Antibody
Antibody SKU:	CAB5452
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human PSMB10 (NP_002792.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	THP-1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human PSMB10 (NP_002792.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLKP ALEP RGGF SFEN CQRN ASLE RVLP GLKV PHAR KTGT TIAG LVFQ DGVI LGAD TRAT NDSV VADK SCEK IHFI APKI YCCG AGVA ADAE MTTR MVAS KMEL HALS TGRE PRVA TVTR ILRQ TLFR YQGH VGAS LIVG GVDL TGPQ LYGV HPHG SYSR LPFT ALGS GQDA ALAV LEDR FQPN MTLE AAQG LLVE AVTA GILG DLGS GGNV DACV ITKT GAKL LRTL SSPT EPVK RSGR YHFV PGTT AVLT QTVK PLTL ELVE ETVQ AMEV E
Gene ID:	5699
Uniprot:	P40306
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	28kDa
Observed MW:	29kDa

Synonyms:

PSMB10, LMP10, MECL1, beta2i

Background:

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.

UniProt Protein Function:	PSMB10: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Belongs to the peptidase T1B family.
UniProt Protein Details:	Protein type:EC 3.4.25.1; Protease; Proteasome complex Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: proteasome complex; nucleoplasm; proteasome core complex; cytosol Molecular Function:threonine endopeptidase activity Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; cell morphogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; humoral immune response; T cell proliferation; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis
NCBI Summary:	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. [provided by RefSeq, Jul 2008]
UniProt Code:	P40306
NCBI GenInfo Identifier:	730376
NCBI Gene ID:	5699
NCBI Accession:	P40306.1
UniProt Secondary Accession:	P40306,Q5U098, B2R5J4,
UniProt Related Accession:	P40306
Molecular Weight:	273
NCBI Full Name:	Proteasome subunit beta type-10
NCBI Synonym Full Names:	proteasome (prosome, macropain) subunit, beta type, 10
NCBI Official Symbol:	PSMB10
NCBI Official Synonym Symbols:	LMP10; MECL1; beta2i
NCBI Protein Information:	proteasome subunit beta type-10; proteasome MECl-1; macropain subunit MECl-1; proteasome subunit MECL1; proteasome subunit beta 7i; proteasome subunit beta-2i; low molecular mass protein 10; proteasome catalytic subunit 2i; multicatalytic endopeptidase complex subunit MECl-1
UniProt Protein Name:	Proteasome subunit beta type-10
UniProt Synonym Protein Names:	Low molecular mass protein 10; Macropain subunit MECl-1; Multicatalytic endopeptidase complex subunit MECl-1; Proteasome MECl-1; Proteasome subunit beta-2i
Protein Family:	Proteasome
UniProt Gene Name:	PSMB10
UniProt Entry Name:	PSB10_HUMAN

	Western blot analysis of extracts of THP-1 cells, using PSMB10 antibody (CAB5452) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat brain using PSMB10 antibody (CAB5452) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using PSMB10 antibody (CAB5452) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of HeLa cells using PSMB10 antibody (CAB5452). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
PSMB10 Antibody (PACO11530)	PSMB10 (Proteasome subunit beta type-10) ELISA Kit (HUFI07016)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)