Anti-PSMC3 Antibody (CAB13345)

Antibody Name:	Anti-PSMC3 Antibody
Antibody SKU:	CAB13345
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human PSMC3 (NP_002799.3).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human
Positive Samples:	MCF7, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human PSMC3 (NP_002799.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEEG GRDK APVQ PQQS PAAA PGGT DEKP SGKE RRDA GDKD KEQE LSEE DKQL QDEL EMLV ERLG EKDT SLYR PALE ELRR QIRS STTS MTSV PKPL KFLR PHYG KLKE IYEN MAPG ENKR FAAD IISV LAMT MSGE RECL KYRL VGSQ EELA SWGH EYVR HLAG EVAK EWQE LDDA EKVQ
Gene ID:	5702
Uniprot:	P17980
Cellular Location:	Cytoplasm, Nucleus, P-body
Calculated MW:	49kDa
Observed MW:	50kDa

Synonyms:

PSMC3, TBP1, ATPase 3

Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9.

UniProt Protein Function:	RPT5: The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. In case of HIV-1 infection, suppresses Tat-mediated transactivation. Belongs to the AAA ATPase family.
UniProt Protein Details:	Protein type:Proteasome complex; Transcription, coactivator/corepressor; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: proteasome complex; nucleoplasm; membrane; perinuclear region of cytoplasm; nucleus; cytosol Molecular Function:protein binding; ATPase activity; transcription coactivator activity; transcription corepressor activity; ATP binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; blastocyst development; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis
NCBI Summary:	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. [provided by RefSeq, Jul 2008]
UniProt Code:	P17980
NCBI GenInfo Identifier:	20532406
NCBI Gene ID:	5702
NCBI Accession:	P17980.3
UniProt Secondary Accession:	P17980,Q3B757, Q3B865, Q53HU5, Q6GPG8, Q6IBS1, Q96HD3 B2R8V1,
UniProt Related Accession:	P17980
Molecular Weight:	439
NCBI Full Name:	26S protease regulatory subunit 6A
NCBI Synonym Full Names:	proteasome (prosome, macropain) 26S subunit, ATPase, 3
NCBI Official Symbol:	PSMC3
NCBI Official Synonym Symbols:	TBP1
NCBI Protein Information:	26S protease regulatory subunit 6A; TBP-1; Tat-binding protein 1; proteasome subunit P50; proteasome 26S subunit ATPase 3; 26S proteasome AAA-ATPase subunit RPT5; human immunodeficiency virus tat transactivator binding protein-1
UniProt Protein Name:	26S protease regulatory subunit 6A
UniProt Synonym Protein Names:	26S proteasome AAA-ATPase subunit RPT5; Proteasome 26S subunit ATPase 3; Proteasome subunit P50; Tat-binding protein 1; TBP-1
Protein Family:	26S protease regulatory
UniProt Gene Name:	PSMC3
UniProt Entry Name:	PRS6A_HUMAN

	Western blot analysis of extracts of various cell lines, using PSMC3 antibody (CAB13345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of U2OS cells using PSMC3 antibody (CAB13345). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug PSMC3 antibody (CAB13345). Western blot was performed from the immunoprecipitate using PSMC3 antibody (CAB13345) at a dilition of 1:1000.

Antibodies	ELISA Kits
Anti-PSMC3 Antibody (CAB1986)	Proteasome alpha3 Colorimetric Cell-Based ELISA Kit
PSMC3 Antibody (PACO04792)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)