Anti-PSMD7 Antibody (CAB5356)

Antibody Name:	Anti-PSMD7 Antibody
Antibody SKU:	CAB5356
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-324 of human PSMD7 (NP_002802.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	MCF7, SW620, A-549, K-562, BT-474, Mouse skeletal muscle, Mouse heart, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-324 of human PSMD7 (NP_002802.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPEL AVQK VVVH PLVL LSVV DHFN RIGK VGNQ KRVV GVLL GSWQ KKVL DVSN SFAV PFDE DDKD DSVW FLDH DYLE NMYG MFKK VNAR ERIV GWYH TGPK LHKN DIAI NELM KRYC PNSV LVII DVKP KDLG LPTE AYIS VEEV HDDG TPTS KTFE HVTS EIGA EEAE EVGV EHLL RDIK DTTV GTLS QRIT NQVH GLKG LNSK LLDI RSYL EKVA TGKL PINH QIIY QLQD VFNL LPDV SLQE FVKA FYLK TNDQ MVVV YLAS LIRS VVAL HNLI NNKI ANRD AEKK EGQE KEES KKDR KEDK EKDK DKEK SDVK KEEK KEKK
Gene ID:	5713
Uniprot:	P51665
Cellular Location:
Calculated MW:	37kDa
Observed MW:	37kDa

Synonyms:

PSMD7, MOV34, P40, Rpn8, S12

Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 17.

UniProt Protein Function:	PSMD7: Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Belongs to the peptidase M67A family.
UniProt Protein Details:	Protein type:Proteasome complex; Protease Chromosomal Location of Human Ortholog: 16q22.3 Cellular Component: proteasome complex; nucleoplasm; membrane; proteasome regulatory particle; nucleus; cytosol Molecular Function:protein binding; protein homodimerization activity Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
NCBI Summary:	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 17. [provided by RefSeq, Jul 2008]
UniProt Code:	P51665
NCBI GenInfo Identifier:	20532412
NCBI Gene ID:	5713
NCBI Accession:	P51665.2
UniProt Secondary Accession:	P51665,Q6PKI2, Q96E97, D3DWS9,
UniProt Related Accession:	P51665
Molecular Weight:	324
NCBI Full Name:	26S proteasome non-ATPase regulatory subunit 7
NCBI Synonym Full Names:	proteasome (prosome, macropain) 26S subunit, non-ATPase, 7
NCBI Official Symbol:	PSMD7
NCBI Official Synonym Symbols:	P40; S12; Rpn8; MOV34
NCBI Protein Information:	26S proteasome non-ATPase regulatory subunit 7; Mov34 homolog; proteasome subunit p40; 26S proteasome regulatory subunit S12; 26S proteasome regulatory subunit rpn8; Moloney leukemia virus-34 proviral integration; proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog)
UniProt Protein Name:	26S proteasome non-ATPase regulatory subunit 7
UniProt Synonym Protein Names:	26S proteasome regulatory subunit RPN8; 26S proteasome regulatory subunit S12; Mov34 protein homolog; Proteasome subunit p40
Protein Family:	26S proteasome non-ATPase regulatory
UniProt Gene Name:	PSMD7
UniProt Entry Name:	PSMD7_HUMAN

	Western blot analysis of extracts of various cell lines, using PSMD7 antibody (CAB5356) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded mouse spleen using PSMD7 antibody (CAB5356) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using PSMD7 antibody (CAB5356). Blue: DAPI for nuclear staining.

Antibodies

PSMD7 Antibody (PACO11555)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)