Anti-PSME3 Antibody (CAB12697)

Antibody Name:	Anti-PSME3 Antibody
Antibody SKU:	CAB12697
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human PSME3 (NP_005780.2).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, Raji, Jurkat, A-549, Mouse spleen, Mouse brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human PSME3 (NP_005780.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KVFV MPNG MLKS NQQL VDII EKVK PEIR LLIE KCNT VKMW VQLL IPRI EDGN NFGV SIQE ETVA ELRT VESE AASY LDQI SRYY ITRA KLVS KIAK YPHV EDYR RTVT EIDE KEYI SLRL IISE LRNQ YVTL HDMI LKNI EKIK RPRS SNAE TLY
Gene ID:	10197
Uniprot:	P61289
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	29kDa/30kDa
Observed MW:	35kDa

Synonyms:

PSME3, HEL-S-283, Ki, PA28-gamma, PA28G, PA28gamma, REG-GAMMA

Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	PSME3: a protein implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. Homohexamer. Significantly elevated in colorectal cancer patients compared with healthy donors and patients with benign bowel disease. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protease; Nuclear receptor co-regulator; Proteasome complex Chromosomal Location of Human Ortholog: 17q21 Cellular Component: proteasome complex; nucleoplasm; proteasome activator complex; membrane; cytoplasm; nucleus; cytosol Molecular Function:protein binding; p53 binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
NCBI Summary:	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012]
UniProt Code:	P61289
NCBI GenInfo Identifier:	47117724
NCBI Gene ID:	10197
NCBI Accession:	P61289.1
UniProt Secondary Accession:	P61289,O35563, P97373, Q12920, Q13172, Q9BQD9, A8K9A3
UniProt Related Accession:	P61289
Molecular Weight:	254
NCBI Full Name:	Proteasome activator complex subunit 3
NCBI Synonym Full Names:	proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
NCBI Official Symbol:	PSME3
NCBI Official Synonym Symbols:	Ki; PA28G; HEL-S-283; PA28gamma; REG-GAMMA; PA28-gamma
NCBI Protein Information:	proteasome activator complex subunit 3; Ki antigen; REG gamma-3; PA28 gamma variant 5; Ki nuclear autoantigen; proteasome activator 28-gamma; 11S regulator complex gamma subunit; 11S regulator complex subunit gamma; epididymis secretory protein Li 283; proteasome activator 28 subunit gamma; activator of multicatalytic protease subunit 3
UniProt Protein Name:	Proteasome activator complex subunit 3
UniProt Synonym Protein Names:	11S regulator complex subunit gamma; REG-gamma; Activator of multicatalytic protease subunit 3; Ki nuclear autoantigen; Proteasome activator 28 subunit gamma; PA28g; PA28gamma
Protein Family:	Proteasome activator
UniProt Gene Name:	PSME3
UniProt Entry Name:	PSME3_HUMAN

	Western blot analysis of extracts of various cell lines, using PSME3 antibody (CAB12697) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of C6 cells using PSME3 Polyclonal Antibody (CAB12697) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using PSME3 Polyclonal Antibody (CAB12697) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using PSME3 Polyclonal Antibody (CAB12697) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-PSME3 Antibody (CAB0271)

Anti-PSME3 Antibody (CAB18021)[KO Validated]

PSME3 Antibody (PACO11558)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)