Anti-PSME3 Antibody (CAB18021)[KO Validated]
- SKU:
- CAB18021
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-PSME3 Antibody [KO Validated] |
| Antibody SKU: | CAB18021 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human PSME3 (NP_005780.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | 293T |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human PSME3 (NP_005780.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KVFV MPNG MLKS NQQL VDII EKVK PEIR LLIE KCNT VKMW VQLL IPRI EDGN NFGV SIQE ETVA ELRT VESE AASY LDQI SRYY ITRA KLVS KIAK YPHV EDYR RTVT EIDE KEYI SLRL IISE LRNQ YVTL HDMI LKNI EKIK RPRS SNAE TLY |
| Gene ID: | 10197 |
| Uniprot: | P61289 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 29kDa/30kDa |
| Observed MW: | 35kDa |
![Anti-PSME3 Antibody [KO Validated] (CAB18021)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/j/200/A18021_1__49061.jpg) | Western blot analysis of extracts from normal (control) and PSME3 knockout (KO) 293T cells, using PSME3 antibody (CAB18021) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min. |
| Synonyms: | PSME3, HEL-S-283, Ki, PA28-gamma, PA28G, PA28gamma, REG-GAMMA |
| Background: | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants. |
| UniProt Protein Function: | PSME3: a protein implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. Homohexamer. Significantly elevated in colorectal cancer patients compared with healthy donors and patients with benign bowel disease. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Protease; Nuclear receptor co-regulator; Proteasome complex Chromosomal Location of Human Ortholog: 17q21 Cellular Component: proteasome complex; nucleoplasm; proteasome activator complex; membrane; cytoplasm; nucleus; cytosol Molecular Function:protein binding; p53 binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle |
| NCBI Summary: | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012] |
| UniProt Code: | P61289 |
| NCBI GenInfo Identifier: | 47117724 |
| NCBI Gene ID: | 10197 |
| NCBI Accession: | P61289.1 |
| UniProt Secondary Accession: | P61289,O35563, P97373, Q12920, Q13172, Q9BQD9, A8K9A3 |
| UniProt Related Accession: | P61289 |
| Molecular Weight: | 254 |
| NCBI Full Name: | Proteasome activator complex subunit 3 |
| NCBI Synonym Full Names: | proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki) |
| NCBI Official Symbol: | PSME3Â Â |
| NCBI Official Synonym Symbols: | Ki; PA28G; HEL-S-283; PA28gamma; REG-GAMMA; PA28-gamma  |
| NCBI Protein Information: | proteasome activator complex subunit 3; Ki antigen; REG gamma-3; PA28 gamma variant 5; Ki nuclear autoantigen; proteasome activator 28-gamma; 11S regulator complex gamma subunit; 11S regulator complex subunit gamma; epididymis secretory protein Li 283; proteasome activator 28 subunit gamma; activator of multicatalytic protease subunit 3 |
| UniProt Protein Name: | Proteasome activator complex subunit 3 |
| UniProt Synonym Protein Names: | 11S regulator complex subunit gamma; REG-gamma; Activator of multicatalytic protease subunit 3; Ki nuclear autoantigen; Proteasome activator 28 subunit gamma; PA28g; PA28gamma |
| Protein Family: | Proteasome activator |
| UniProt Gene Name: | PSME3Â Â |
| UniProt Entry Name: | PSME3_HUMAN |
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