Anti-PSMF1 Antibody (CAB5554)

Antibody Name:	Anti-PSMF1 Antibody
Antibody SKU:	CAB5554
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-271 of human PSMF1 (NP_006805.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, A-549, Mouse liver, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-271 of human PSMF1 (NP_006805.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGL EVLF ASAA PAIT CRQD ALVC FLHW EVVT HGYF GLGV GDQP GPND KKSE LLPA GWNN NKDL YVLR YEYK DGSR KLLV KAIT VESS MILN VLEY GSQQ VADL TLNL DDYI DAEH LGDF HRTY KNSE ELRS RIVS GIIT PIHE QWEK ANVS SPHR EFPP ATAR EVDP LRIP PHHP HTSR QPPW CDPL GPFV VGGE DLDP FGPR RGGM IVDP LRSG FPRA LIDP SSGL PNRL PPGA VPPG ARFD PFGP IGTS PPGP NPDH LPPP GYDD MYL
Gene ID:	9491
Uniprot:	Q92530
Cellular Location:	Cytoplasm, Endoplasmic reticulum
Calculated MW:	29kDa
Observed MW:	35kDa

Synonyms:

PSMF1, PI31

Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.

UniProt Protein Function:	PSMF1: Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28. Belongs to the proteasome inhibitor PI31 family.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 20p13 Cellular Component: nucleoplasm; proteasome core complex; membrane; endoplasmic reticulum; cytoplasm; cytosol Molecular Function:protein binding; endopeptidase inhibitor activity Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis
NCBI Summary:	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q92530
NCBI GenInfo Identifier:	134047876
NCBI Gene ID:	9491
NCBI Accession:	Q92530.2
UniProt Secondary Accession:	Q92530,Q9H4I1, A0AVQ9, D3DVW3,
UniProt Related Accession:	Q92530
Molecular Weight:	29,817 Da
NCBI Full Name:	Proteasome inhibitor PI31 subunit
NCBI Synonym Full Names:	proteasome (prosome, macropain) inhibitor subunit 1 (PI31)
NCBI Official Symbol:	PSMF1
NCBI Official Synonym Symbols:	PI31
NCBI Protein Information:	proteasome inhibitor PI31 subunit; hPI31; proteasome inhibitor hP131 subunit
UniProt Protein Name:	Proteasome inhibitor PI31 subunit
Protein Family:	Proteasome inhibitor
UniProt Gene Name:	PSMF1
UniProt Entry Name:	PSMF1_HUMAN

	Western blot analysis of extracts of various cell lines, using PSMF1 Antibody (CAB5554) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver damage using PSMF1 antibody (CAB5554) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat testis using PSMF1 antibody (CAB5554) at dilution of 1:50 (40x lens).

Antibodies

PSMF1 Antibody (PACO11560)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)