Anti-PTP4A2 Antibody (CAB15335)

Antibody Name:	Anti-PTP4A2 Antibody
Antibody SKU:	CAB15335
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-100 of human PTP4A2 (NP_536316.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, Mouse skeletal muscle, Mouse brain, Mouse kidney, Mouse liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-100 of human PTP4A2 (NP_536316.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FTEE LKKY GVTT LVRV CDAT YDKA PVEK EGIH VLDW PFDD GAPP PNQI VDDW LNLL KTKF REEP GCCV AVH
Gene ID:	8073
Uniprot:	Q12974
Cellular Location:	Cell membrane, Cytoplasm, Early endosome
Calculated MW:	9kDa/15kDa/16kDa/19kDa
Observed MW:	19kDa

Synonyms:

PTP4A2, HH13, HH7-2, HU-PP-1, OV-1, PRL-2, PRL2, PTP4A, PTPCAAX2, ptp-IV1a, ptp-IV1b

Background:

The protein encoded by this gene belongs to a small class of the protein tyrosine phosphatase (PTP) family. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. PTPs in this class contain a protein tyrosine phosphatase catalytic domain and a characteristic C-terminal prenylation motif. This PTP has been shown to primarily associate with plasmic and endosomal membrane through its C-terminal prenylation. This PTP was found to interact with the beta-subunit of Rab geranylgeranyltransferase II (beta GGT II), and thus may function as a regulator of GGT II activity. Overexpression of this gene in mammalian cells conferred a transformed phenotype, which suggested its role in tumorigenesis. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 11, 12 and 17.

UniProt Protein Function:	PTP4A2: Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Promotes tumors. Inhibits geranylgeranyl transferase type II activity by blocking the association between RABGGTA and RABGGTB. Belongs to the protein-tyrosine phosphatase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor); Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1p35 Cellular Component: cytoplasm; nucleus Molecular Function:prenylated protein tyrosine phosphatase activity
NCBI Summary:	The protein encoded by this gene belongs to a small class of the protein tyrosine phosphatase (PTP) family. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. PTPs in this class contain a protein tyrosine phosphatase catalytic domain and a characteristic C-terminal prenylation motif. This PTP has been shown to primarily associate with plasmic and endosomal membrane through its C-terminal prenylation. This PTP was found to interact with the beta-subunit of Rab geranylgeranyltransferase II (beta GGT II), and thus may function as a regulator of GGT II activity. Overexpression of this gene in mammalian cells conferred a transformed phenotype, which suggested its role in tumorigenesis. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 11, 12 and 17. [provided by RefSeq, Aug 2010]
UniProt Code:	Q12974
NCBI GenInfo Identifier:	68566159
NCBI Gene ID:	8073
NCBI Accession:	Q12974.1
UniProt Secondary Accession:	Q12974,O00649, Q15197, Q15259, Q15260, Q15261, A8K9I8 B4DM39, D3DPP0, E9PGJ6, R4GN50,
UniProt Related Accession:	Q12974
Molecular Weight:	16,367 Da
NCBI Full Name:	Protein tyrosine phosphatase type IVA 2
NCBI Synonym Full Names:	protein tyrosine phosphatase type IVA, member 2
NCBI Official Symbol:	PTP4A2
NCBI Official Synonym Symbols:	HH13; OV-1; PRL2; HH7-2; PRL-2; PTP4A; HU-PP-1; PTPCAAX2; ptp-IV1a; ptp-IV1b
NCBI Protein Information:	protein tyrosine phosphatase type IVA 2
UniProt Protein Name:	Protein tyrosine phosphatase type IVA 2
UniProt Synonym Protein Names:	HU-PP-1; OV-1; PTP(CAAXII); Protein-tyrosine phosphatase 4a2; Protein-tyrosine phosphatase of regenerating liver 2; PRL-2
Protein Family:	Protein tyrosine phosphatase
UniProt Gene Name:	PTP4A2
UniProt Entry Name:	TP4A2_HUMAN

	Western blot analysis of extracts of various cell lines, using PTP4A2 antibody (CAB15335) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat kidney using PTP4A2 antibody (CAB15335) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using PTP4A2 antibody (CAB15335) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using PTP4A2 antibody (CAB15335) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using PTP4A2 antibody (CAB15335) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)