Anti-PTPRA Antibody (CAB1976)

Antibody Name:	Anti-PTPRA Antibody
Antibody SKU:	CAB1976
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-142 of human PTPRA (NP_543031.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	SW620, 22Rv1, BT-474, Mouse brain, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-142 of human PTPRA (NP_543031.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NNAT TVAP SVGI TRLI NSST AEPV KEEA KTSN PTSS LTSL SVAP TFSP NITL GPTY LTTV NSSD SDNG TTRT ASTN SIGI TISP NGTW LPDN QFTD ARTE PWEG NSST AATT PETF PPSD ETP
Gene ID:	5786
Uniprot:	P18433
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	89kDa/90kDa
Observed MW:	120kDa

Synonyms:

PTPRA, HEPTP, HLPR, HPTPA, HPTPalpha, LRP, PTPA, PTPRL2, R-PTP-alpha, RPTPA

Background:

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. This PTP has been shown to dephosphorylate and activate Src family tyrosine kinases, and is implicated in the regulation of integrin signaling, cell adhesion and proliferation. Three alternatively spliced variants of this gene, which encode two distinct isoforms, have been reported.

UniProt Protein Function:	PTPRA: a receptor protein tyrosine phosphatase (PTP). Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. Dephosphorylates and activates Src family tyrosine kinases, and is implicated in the regulation of integrin signaling, cell adhesion and proliferation. Four alternatively spliced isoforms have been reported.
UniProt Protein Details:	Protein type:Membrane protein, integral; Receptor protein phosphatase, tyrosine; Motility/polarity/chemotaxis; EC 3.1.3.48 Chromosomal Location of Human Ortholog: 20p13 Cellular Component: integral to plasma membrane; plasma membrane; receptor complex Molecular Function:protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity Biological Process: axon guidance; insulin receptor signaling pathway; protein amino acid phosphorylation
NCBI Summary:	The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. This PTP has been shown to dephosphorylate and activate Src family tyrosine kinases, and is implicated in the regulation of integrin signaling, cell adhesion and proliferation. Three alternatively spliced variants of this gene, which encode two distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:	P18433
NCBI GenInfo Identifier:	126467
NCBI Gene ID:	5786
NCBI Accession:	P18433.2
UniProt Secondary Accession:	P18433,Q14513, Q7Z2I2, Q96TD9, A8K2G8, D3DVX5,
UniProt Related Accession:	P18433
Molecular Weight:	802
NCBI Full Name:	Receptor-type tyrosine-protein phosphatase alpha
NCBI Synonym Full Names:	protein tyrosine phosphatase, receptor type, A
NCBI Official Symbol:	PTPRA
NCBI Official Synonym Symbols:	LRP; HLPR; PTPA; HEPTP; HPTPA; RPTPA; PTPRL2; HPTPalpha; R-PTP-alpha
NCBI Protein Information:	receptor-type tyrosine-protein phosphatase alpha; PTPase-alpha; PTPLCA-related phosphatase; tyrosine phosphatase alpha; protein-tyrosine phosphatase alpha; protein tyrosine phosphatase, receptor type, alpha polypeptide; Leukocyte common antigen-related peptide (protein tyrosine phosphate)
UniProt Protein Name:	Receptor-type tyrosine-protein phosphatase alpha
Protein Family:	Receptor-type tyrosine-protein phosphatase
UniProt Gene Name:	PTPRA
UniProt Entry Name:	PTPRA_HUMAN

	Western blot analysis of extracts of various cell lines, using PTPRA antibody (CAB1976) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon using PTPRA Antibody (CAB1976) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney using PTPRA Antibody (CAB1976) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using PTPRA Antibody (CAB1976) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-PTPRA Antibody (CAB12318)	Human Receptor-type tyrosine-protein phosphatase alpha (PTPRA) ELISA Kit
Anti-Phospho-PTPRA-Y789 pAb Antibody (CABP0814)
Phospho-PTPRA (Y798) Antibody (PACO04841)
PTPRA Antibody (PACO04842)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)