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Western blot analysis of extracts of various cell lines, using PWP1 antibody (CAB21143) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features

PWP1 Monoclonal Antibody (CAB21143)

Datasheet MSDS
SKU CAB21143
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human
Host Species Rabbit
Synonyms NCLB, IEF-SSP-9502, PWP1
€131 - €457

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Description

system_update_altDatasheetsystem_update_altMSDS

PWP1 Monoclonal Antibody (CAB21143)

The PWP1 Monoclonal Antibody (CAB21143) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from mouse hybridoma cells, exhibits high specificity and sensitivity for detecting PWP1 in various biological samples, including human tissues. It has been rigorously validated for use in techniques such as immunofluorescence, immunohistochemistry, and flow cytometry.PWP1, also known as regulatory subunit of protein phosphatase 1, is essential for the maintenance of genomic stability and the control of gene expression.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:PWP1 Monoclonal Antibody
SKU:CAB21143
Size:20μL, 100μL
Reactivity:Human
Clone Number:ARC3017
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:ISTV SSVN EAFG RRER LVLG SARN SSIS GPFG SRSS DTPM ES
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:NCLB, IEF-SSP-9502, PWP1
Positive Sample:HeLa, Jurkat
Cellular Localization:Nucleus.
Calculated MW:56kDa
Observed MW:75kDa

The protein encoded by this gene contains several WD-40 repeats and is found mostly in the nucleus. The expression and localization of this protein are cell cycle dependent. Expression of this gene is upregulated in pancreatic adenocarcinoma. Three transcript variants encoding different isoforms have been found for this gene.

Purification MethodAffinity purification
Gene ID11137
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using PWP1 Rabbit mAb (CAB21143) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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