Anti-QARS Antibody (CAB6960)

Antibody Name:	Anti-QARS Antibody
Antibody SKU:	CAB6960
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human QARS (NP_005042.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	HL-60, 293T, HeLa, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human QARS (NP_005042.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAL DSLS LFTS LGLS EQKA RETL KNSA LSAQ LREA ATQA QQTL GSTI DKAT GILL YGLA SRLR DTRR LSFL VSYI ASKK IHTE PQLS AALE YVRS HPLD PIDT VDFE RECG VGVI VTPE QIEE AVEA AINR HRPQ LLVE RYHF NMGL LMGE ARAV LKWA DGKM IKNE VDMQ VLHL LGPK LEAD LEKK FKVA KARL EETD RRTA KDVV ENGE TADQ TLSL MEQL RGEA LKFH KPGE NYKT PGYV VTPH TM
Gene ID:	5859
Uniprot:	P47897
Cellular Location:	Cytoplasm
Calculated MW:	86kDa/87kDa
Observed MW:	88kDa

Synonyms:

QARS, GLNRS, MSCCA, PRO2195

Background:

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. In metazoans, 9 aminoacyl-tRNA synthetases specific for glutamine (gln), glutamic acid (glu), and 7 other amino acids are associated within a multienzyme complex. Although present in eukaryotes, glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	QARS: catalyzes the formation of L-glutaminyl-tRNA. Belongs to the class-I aminoacyl-tRNA synthetase family.
UniProt Protein Details:	Protein type:Ligase; EC 6.1.1.18; Mitochondrial; Translation Chromosomal Location of Human Ortholog: 3p21.31 Cellular Component: mitochondrial matrix; cytoplasm; cytosol Molecular Function:protein binding; glutamine-tRNA ligase activity; ATP binding Biological Process: tRNA aminoacylation for protein translation; glutaminyl-tRNA aminoacylation; gene expression; brain development Disease: Microcephaly, Progressive, With Seizures And Cerebral And Cerebellar Atrophy
NCBI Summary:	Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. In metazoans, 9 aminoacyl-tRNA synthetases specific for glutamine (gln), glutamic acid (glu), and 7 other amino acids are associated within a multienzyme complex. Although present in eukaryotes, glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
UniProt Code:	P47897
NCBI GenInfo Identifier:	1351170
NCBI Gene ID:	5859
NCBI Accession:	P47897.1
UniProt Secondary Accession:	P47897,B4DWJ2,
UniProt Related Accession:	P47897
Molecular Weight:	775
NCBI Full Name:	Glutamine--tRNA ligase
NCBI Synonym Full Names:	glutaminyl-tRNA synthetase
NCBI Official Symbol:	QARS
NCBI Official Synonym Symbols:	GLNRS; MSCCA; PRO2195
NCBI Protein Information:	glutamine--tRNA ligase; glutamine-tRNA synthetase
UniProt Protein Name:	Glutamine--tRNA ligase
UniProt Synonym Protein Names:	Glutaminyl-tRNA synthetase; GlnRS
UniProt Gene Name:	QARS
UniProt Entry Name:	SYQ_HUMAN

	Western blot analysis of extracts of various cell lines, using QARS antibody (CAB6960) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using QARS antibody (CAB6960) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver damage using QARS antibody (CAB6960) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using QARS antibody (CAB6960) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using QARS antibody (CAB6960). Blue: DAPI for nuclear staining.

Antibodies

QARS Antibody (PACO11624)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)