Anti-QPRT Antibody (CAB14349)

Antibody Name:	Anti-QPRT Antibody
Antibody SKU:	CAB14349
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human QPRT (NP_055113.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human QPRT (NP_055113.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDAE GLAL LLPP VTLA ALVD SWLR EDCP GLNY AALV SGAG PSQA ALWA KSPG VLAG QPFF DAIF TQLN CQVS WFLP EGSK LVPV ARVA EVRG PAHC LLLG ERVA LNTL ARCS GIAS AAAA AVEA ARGA GWTG HVAG TRKT TPGF RLVE KYGL LVGG AASH RYDL GGLV MVKD NHVV AAGG VEKA VRAA RQAA DFAL KVEV ECSS LQEA VQAA EAGA DLVL LDNF KPEE LHPT ATVL KAQF PSVA VEAS GGIT LDNL PQFC GPHI DVIS MGML TQAA PALD FSLK LFAK EVAP VPKI H
Gene ID:	23475
Uniprot:	Q15274
Cellular Location:
Calculated MW:	30kDa
Observed MW:	31kDa

Synonyms:

QPRT, HEL-S-90n, QPRTase

Background:

This gene encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	QPRT: Involved in the catabolism of quinolinic acid (QA). Belongs to the NadC/ModD family.
UniProt Protein Details:	Protein type:Transferase; EC 2.4.2.19; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: cytoplasm; cytosol Molecular Function:nicotinate-nucleotide diphosphorylase (carboxylating) activity; protein homodimerization activity Biological Process: NAD biosynthetic process; NAD metabolic process; protein oligomerization
NCBI Summary:	This gene encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
UniProt Code:	Q15274
NCBI GenInfo Identifier:	296439291
NCBI Gene ID:	23475
NCBI Accession:	Q15274.3
UniProt Secondary Accession:	Q15274,Q53XW7, Q96G22, Q9BSG6,
UniProt Related Accession:	Q15274
Molecular Weight:	30,846 Da
NCBI Full Name:	Nicotinate-nucleotide pyrophosphorylase
NCBI Synonym Full Names:	quinolinate phosphoribosyltransferase
NCBI Official Symbol:	QPRT
NCBI Official Synonym Symbols:	QPRTase; HEL-S-90n
NCBI Protein Information:	nicotinate-nucleotide pyrophosphorylase [carboxylating]
UniProt Protein Name:	Nicotinate-nucleotide pyrophosphorylase [carboxylating]
UniProt Synonym Protein Names:	Quinolinate phosphoribosyltransferase [decarboxylating]; QAPRTase; QPRTase
Protein Family:	Nicotinate-nucleotide pyrophosphorylase
UniProt Gene Name:	QPRT
UniProt Entry Name:	NADC_HUMAN

Western blot analysis of extracts of various cell lines, using QPRT antibody (CAB14349) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

ELISA Kits

Human QPRT (Quinolinate phosphoribosyltransferase) ELISA Kit (HUFI08578)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)