The QuickStep Bovine PG (Progesterone) ELISA Kit is specifically designed for the sensitive and precise measurement of progesterone levels in bovine serum, plasma, and other biological samples. This cutting-edge kit offers exceptional accuracy and reliability, ensuring dependable results for a variety of research applications.Progesterone is a key hormone in the reproductive system of cattle, playing a crucial role in regulating the estrous cycle and supporting successful pregnancies. Monitoring progesterone levels in bovine samples is essential for understanding reproductive health, optimizing breeding practices, and improving overall herd management.
With its advanced technology and user-friendly protocol, the QuickStep Bovine PG ELISA Kit is a valuable tool for researchers, veterinarians, and agricultural professionals alike. Trust in this kit to deliver accurate and consistent progesterone measurements, driving impactful research and innovation in the field of bovine reproductive biology.
Product Name:
QuickStep Bovine Progesterone ELISA Kit
Product Code:
QSES005
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Bovine
Sensitivity:
23.24 pg/mL
Range:
78.13-5000 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Progesterone
Application:
This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum and plasma.
Specificity:
This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Bovine plasma samples and diluted Bovine serum samples at 5-10 fold .
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Pg are added to the micro ELISA plate wells. Bovine Pg in samples (or standards) competes with a fixed amount of Bovine Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Bovine Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Bovine Pg in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
240.71
569.79
2369.60
226.64
611.90
2495.08
Standard deviation
14.47
27.18
129.14
15.68
29.25
118.27
CV (%)
6.01
4.77
5.45
6.92
4.78
4.74
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
92-106
100
EDTA plasma(n=8)
85-98
91
Samples were spiked with high concentrations of Bovine Pg and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
