The QuickStep Porcine PG (Progesterone) ELISA Kit is specifically designed for the accurate and reliable detection of porcine progesterone levels in serum, plasma, and other sample types. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Progesterone is a key hormone in regulating the female reproductive system, playing a crucial role in the menstrual cycle, pregnancy, and other reproductive functions. Monitoring progesterone levels is essential for studying reproductive disorders, fertility issues, and assessing pregnancy status in pigs.
Whether you are studying reproductive physiology, conducting fertility research, or working on animal breeding programs, the QuickStep Porcine PG ELISA Kit provides a valuable tool for measuring porcine progesterone levels accurately and efficiently. Trust in this kit for robust and trustworthy results to advance your research in the field of porcine reproductive health.
Product Name:
QuickStep Porcine Progesterone ELISA Kit
Product Code:
QSES047
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Porcine
Sensitivity:
24.38 pg/mL
Range:
62.50-4000 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Progesterone
Application:
This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum and plasma.
Specificity:
This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Porcine serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Porcine Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Porcine Pg are added to the micro ELISA plate wells. Porcine Pg in samples (or standards) competes with a fixed amount of Porcine Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Porcine Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Porcine Pg in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
192.06
508.99
1443.30
182.41
505.75
1518.32
Standard deviation
11.10
23.36
65.24
10.31
25.54
63.92
CV (%)
5.78
4.59
4.52
5.65
5.05
4.21
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
89-102
96
EDTA plasma(n=8)
88-99
93
Samples were spiked with high concentrations of Porcine Pg and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
