The QuickStep Horse E2 (Estradiol) ELISA Kit is a cutting-edge assay designed for the accurate measurement of estradiol levels in equine samples. This kit offers high sensitivity and specificity, delivering precise and consistent results for researchers studying hormonal changes in horses.Estradiol is a key estrogen hormone that regulates various physiological processes in horses, including reproductive health and overall well-being. Monitoring estradiol levels in horses is essential for understanding their reproductive cycles, fertility status, and potential health issues.
With its easy-to-use protocol and reliable performance, the QuickStep Horse E2 ELISA Kit is an indispensable tool for equine researchers, veterinarians, and horse breeders looking to advance their understanding of hormonal regulation in horses and enhance their breeding and management practices.
Product Name:
QuickStep Horse Estradiol ELISA Kit
Product Code:
QSES031
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Horse
Sensitivity:
3.46 pg/mL
Range:
7.81-500 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Estradiol
Application:
This ELISA kit applies to the in vitro quantitative determination of E2 concentrations in serum and plasma.
Specificity:
This kit recognizes E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Horse serum, plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Cortisol are added to the micro ELISA plate wells. Bovine Cortisol in samples (or standards) competes with a fixed amount of Bovine Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Bovine Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Bovine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
27.73
58.23
174.38
25.63
59.05
159.36
Standard deviation
1.78
3.32
7.53
1.53
2.96
7.20
CV (%)
6.43
5.71
4.32
5.98
5.02
4.52
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
95-108
101
EDTA plasma(n=8)
91-106
98
Samples were spiked with high concentrations of Horse E2 and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
