The QuickStep Human PG (Progesterone) ELISA Kit is specifically designed for the precise measurement of progesterone levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees accurate and dependable results, making it perfect for various research purposes.Progesterone is a vital hormone that plays a key role in the female reproductive system, regulating the menstrual cycle and supporting pregnancy. Imbalances in progesterone levels can lead to infertility, miscarriages, and other reproductive health issues.
Therefore, monitoring progesterone levels is crucial in understanding and managing these conditions.Whether you are studying hormonal disorders, fertility issues, or pregnancy complications, the QuickStep Human PG ELISA Kit offers a reliable solution for measuring progesterone levels with precision and confidence. Get your kit today and unlock new insights in your research.
Product Name:
QuickStep Human Progesterone ELISA Kit
Product Code:
QSES025
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Human
Sensitivity:
36.24 pg/mL
Range:
78.13-5000 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Progesterone
Application:
This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum and plasma.
Specificity:
This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Human serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human Pg are added to the micro ELISA plate wells. Human Pg in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human Pg. The concentration of Human Pg in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
247.69
769.94
1676.10
229.30
733.82
1806.27
Standard deviation
13.82
43.27
62.52
12.86
35.66
65.39
CV (%)
5.58
5.62
3.73
5.61
4.86
3.62
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
88-103
95
EDTA plasma(n=8)
96-111
102
Samples were spiked with high concentrations of Human Pg and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
