The QuickStep Mouse Cort Corticosterone ELISA Kit is a cutting-edge assay designed for the precise measurement of corticosterone levels in mouse serum, plasma, and tissue extracts. This kit offers exceptional sensitivity and accuracy, guaranteeing consistent and trustworthy results for a variety of research purposes.Corticosterone is a key steroid hormone that plays a critical role in stress response, metabolism, and immune function. Monitoring corticosterone levels is crucial for investigating stress-related disorders, metabolic diseases, and immune system dysregulation.
The QuickStep Mouse Cort Corticosterone ELISA Kit provides researchers with a powerful tool to study the physiological and pathological implications of corticosterone levels in mouse models.Don't miss out on this advanced ELISA kit for comprehensive analysis of corticosterone in mouse samples. Stay ahead in your research with the QuickStep Mouse Cort Corticosterone ELISA Kit from Assay Genie.
Product Name:
QuickStep Mouse Corticosterone ELISA Kit
Product Code:
QSES032
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Mouse
Sensitivity:
4.98 ng/mL
Range:
12.50-800 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum, plasma, urine and saliva
Target:
Corticosterone
Application:
This ELISA kit applies to the in vitro quantitative determination of Cort concentrations in serum, plasma, urine, saliva.
Specificity:
This kit recognizes Cort in samples. No significant cross-reactivity or interference between Cort and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Mouse serum, plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Cortisol are added to the micro ELISA plate wells. Bovine Cortisol in samples (or standards) competes with a fixed amount of Bovine Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Bovine Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Bovine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
41.41
114.40
374.35
42.38
108.31
346.98
Standard deviation
2.77
5.70
20.03
2.95
5.10
18.08
CV (%)
6.69
4.98
5.35
6.95
4.71
5.21
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
91-106
98
EDTA plasma(n=8)
88-103
94
Samples were spiked with high concentrations of Mouse Cort and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
