QuickStep Mouse IgG3 (Immunoglobulin G3) ELISA Kit
- SKU:
- AEES00662
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Mouse
- Sensitivity:
- 0.14 ng/mL
- Range:
- 0.31-20 ng/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
| Product Name: | QuickStep Mouse IgG3 (Immunoglobulin G3) ELISA Kit |
| Product Code: | AEES00662 |
| Assay Type: | Sandwich |
| Format: | 96 Assays |
| Assay Time: | 1.5h |
| Reactivity: | Mouse |
| Detection Range: | 0.31-20 ng/mL |
| Sensitivity: | 0.14 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 50μL |
| Specificity: | This kit recognizes IgG3 in samples. No significant cross-reactivity or interference between IgG3 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of IgG3 concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgG3 are added to the micro ELISA plate wells. Mouse IgG3 in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to IgG3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Mouse IgG3 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
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