The Mouse IgG3 (Immunoglobulin G3) ELISA Kit is specifically designed for the accurate quantification of IgG3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring dependable and consistent results, perfect for various research purposes.IgG3 is an essential subclass of immunoglobulins that plays a crucial role in the immune response by recognizing and binding to specific antigens, thereby mediating immune effector functions. Elevated levels of IgG3 have been linked to certain autoimmune diseases, infections, and inflammatory conditions, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.Overall, the Mouse IgG3 ELISA Kit provides researchers with a reliable tool for investigating the role of IgG3 in immune responses and disease pathogenesis, ultimately advancing our understanding of these processes and improving diagnostics and treatment strategies.
Product Name:
Mouse IgG3 (Immunoglobulin G3) ELISA Kit
SKU:
MOES01682
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
