The QuickStep Rabbit E2 (Estradiol) ELISA Kit offered by Assay Genie is a powerful tool for accurately measuring estradiol levels in rabbit samples. This kit is highly sensitive and specific, allowing for precise and reproducible results in serum, plasma, and cell culture supernatants.Estradiol, a form of estrogen, plays a crucial role in reproductive health and is involved in various physiological processes. Monitoring estradiol levels is essential for research in areas such as reproductive biology, endocrinology, and hormone-related diseases.
The QuickStep Rabbit E2 ELISA Kit provides researchers with a reliable method for studying the impact of estradiol on various biological pathways and potential therapeutic interventions.Trust Assay Genie's QuickStep Rabbit E2 ELISA Kit for accurate and consistent measurement of estradiol levels in rabbit samples, facilitating valuable insights into reproductive health and hormone-related research.
Product Name:
QuickStep Rabbit Estradiol ELISA Kit
Product Code:
QSES055
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Rabbit
Sensitivity:
5.65 pg/mL
Range:
15.63-1000 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Estradiol
Application:
This ELISA kit applies to the in vitro quantitative determination of E2 concentrations in serum and plasma.
Specificity:
This kit recognizes E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Rabbit serum, plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Cortisol are added to the micro ELISA plate wells. Bovine Cortisol in samples (or standards) competes with a fixed amount of Bovine Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Bovine Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Bovine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
50.21
96.93
495.65
46.35
90.65
490.39
Standard deviation
3.06
4.85
23.30
2.82
4.56
24.23
CV (%)
6.10
5.00
4.70
6.08
5.03
4.94
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
85-97
90
EDTA plasma(n=8)
90-102
97
Samples were spiked with high concentrations of Rabbit E2 and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
