The QuickStep Rat Cort Corticosterone ELISA Kit is a reliable and sensitive assay designed for the quantitative detection of corticosterone levels in rat serum, plasma, and tissue homogenates. This kit offers high specificity and accuracy, ensuring precise and consistent results for your research needs.Corticosterone is a key steroid hormone involved in stress response, metabolism, and immune function. Dysregulation of corticosterone levels has been linked to various physiological and behavioral disorders, making it a valuable marker for studying stress-related conditions and drug development.
With its easy-to-follow protocol and quick assay time, the QuickStep Rat Cort Corticosterone ELISA Kit is an essential tool for researchers aiming to investigate the role of corticosterone in biological processes and disease pathways. Get reliable data and accelerate your research with this cutting-edge ELISA kit.
Product Name:
QuickStep Rat Corticosterone ELISA Kit
Product Code:
QSES049
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Rat
Sensitivity:
1.87 ng/mL
Range:
4.69-300 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum, plasma, urine and saliva
Target:
Corticosterone
Application:
This ELISA kit applies to the in vitro quantitative determination of Cort concentrations in serum, plasma, urine, saliva.
Specificity:
This kit recognizes Cort in samples. No significant cross-reactivity or interference between Cort and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted or 2~5 fold diluted Rat serum, plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Cortisol are added to the micro ELISA plate wells. Bovine Cortisol in samples (or standards) competes with a fixed amount of Bovine Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Bovine Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Bovine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
13.64
37.16
121.75
14.17
37.85
113.64
Standard deviation
0.70
2.02
4.49
0.77
2.27
4.78
CV (%)
5.12
5.43
3.69
5.41
6.00
4.21
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
92-105
97
EDTA plasma(n=8)
91-105
98
Samples were spiked with high concentrations of Rat Cort and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
