The QuickStep Rat E3 Estriol ELISA Kit is specially designed for the precise measurement of E3 Estriol levels in rat serum, plasma, and tissue lysates. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it perfect for various research applications.E3 Estriol is a key hormone that plays a crucial role in pregnancy and is often used as a biomarker for assessing fetal health and development.
Monitoring E3 Estriol levels can provide valuable insights into reproductive health and may aid in the diagnosis and management of pregnancy-related complications.Whether you are studying reproductive physiology, conducting preclinical research, or investigating the effects of hormones on rat models, the QuickStep Rat E3 Estriol ELISA Kit is a valuable tool for accurate and efficient measurement of E3 Estriol levels.
Product Name:
QuickStep Rat Estriol ELISA Kit
Product Code:
QSES051
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Rat
Sensitivity:
7.96 pg/mL
Range:
15.63-1000 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Estriol
Application:
This ELISA kit applies to the in vitro quantitative determination of E3 concentrations in serum and plasma.
Specificity:
This kit recognizes E3 in samples. No significant cross-reactivity or interference between E3 and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Rat serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat E3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat E3 are added to the micro ELISA plate wells. Rat E3 in samples (or standards) competes with a fixed amount of Rat E3 on the solid phase supporter for sites on the HRP linked detection antibody specific to Rat E3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Rat E3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
47.41
83.91
407.09
42.82
87.05
378.33
Standard deviation
3.08
4.84
14.04
2.97
4.00
14.30
CV (%)
6.50
5.77
3.45
6.93
4.59
3.78
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
87-98
93
EDTA plasma(n=8)
93-108
100
Samples were spiked with high concentrations of Rat E3 and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
