The QuickStep Rat IgG (Immunoglobulin G) ELISA Kit is a highly reliable and accurate tool for detecting levels of IgG in rat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides consistent and reproducible results, making it perfect for a wide range of research purposes.IgG is a critical component of the immune system, playing a vital role in fighting off infections and providing immunity. Monitoring IgG levels can help researchers understand immune responses, autoimmune diseases, and allergic reactions in rats, making this kit an essential tool for studying these conditions and developing potential treatments.Overall, the QuickStep Rat IgG ELISA Kit is a valuable resource for researchers looking to analyze and quantify IgG levels in rat samples with precision and accuracy.
Product Name:
Rat IgG (Immunoglobulin G) Quickstep ELISA Kit
SKU:
QSES052
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
1 h 30 min
Sensitivity:
2.98 ng/mL
Detection range:
7.81-500 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
